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Title: Functional characterization of the triple gene block 1 (TGB1) gene of Pepino mosaic virus in tomato

item Padmanabhan, Chellappan
item ZHENG, YI - Boyce Thompson Institute
item Shamimuzzaman, Md
item FEI, ZHANGJUN - Boyce Thompson Institute
item Ling, Kai-Shu

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/5/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary: N/A

Technical Abstract: Pepino mosaic virus (PepMV) has caused serious economic losses to many greenhouse tomato productions around the world. This potexvirus genome contains five major open reading frames (ORFs) encoding for a 164-kDa RNA-dependent RNA polymerase (RdRp), three triple gene block (TGB) proteins of 26, 14 and 9 kDa, and a 25-kDa coat protein, respectively. Although there is some knowledge about the functions for TGB proteins in other potexviruses, the function of the PepMV TGB gene is currently unknown. Using the TGB1 gene from a severe strain of PepMV CH2 genotype, we constructed a plant transformation vector for Agrobacterium-mediated transformation on tomato (cv. Moneymaker). In screening transgenic plants for resistance to PepMV infection, through mechanical inoculation, a number of transgenic tomato plants exhibited resistance to PepMV infection, with a delay in symptom expression over that of GFP- control tomato plants. To investigate the effect of differential gene expression through RNA sequencing between the TGB1 transgenic plants and control GFP-plants upon PepMV infection, we identified a total of 217 differentially expressed genes (DEGs) through transcriptome analysis. Those DEGs and their roles in host gene regulation and virus resistance will be discussed. Understanding the PepMV-encoded TGB1 function might guide us to better utilize the TGB1 gene to manage PepMV infection in greenhouse tomato productions.