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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #339578

Research Project: CHARACTERIZING, DETECTING, AND ELIMINATING PATHOGENS TO ENABLE THE SAFE INTRODUCTION OF PLANT GENETIC RESOURCES

Location: National Germplasm Resources Laboratory

Title: Complete genome sequence of a divergent strain of lettuce chlorosis virus from Periwinkle in China

Author
item TIAN, XIN - Southwest University
item TIAN, YAN - Southwest University
item YU, YUN-QI - Southwest University
item HU, YAN - Southwest University
item LI, ZHONGAN - Southwest University
item Li, Ruhui
item ZHOU, CHANGYONG - Southwest University
item CAO, MENGJI - Southwest University

Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/9/2017
Publication Date: 9/17/2017
Citation: Tian, X., Tian, Y., Yu, Y., Hu, Y., Li, Z., Li, R., Zhou, C., Cao, M. 2017. Complete genome sequence of a divergent strain of lettuce chlorosis virus from Periwinkle in China. Plant Disease. http://apsjournals.apsnet.org/doi/abs/10.1094/PDIS-07-17-1053-PDN.

Interpretive Summary: Periwinkle or Madagascar periwinkle [Catharanthus roseus (L.)], a perennial and evergreen herb in the family Apocynaceae, is a popular ornamental and medicinal plant in subtropical and tropical regions of the world. A number of viruses and virus-like pathogens infect periwinkle, causing various diseases in the crop. In this study, Lettuce chlorosis virus (LCV) was identified by high throughput sequencing from diseased periwinkle plants. The complete genomic sequence of the virus, which consists of two segments of RNA, was determined. Analyses of the genetic information indicate this virus is a novel strain. This is the first report of LCV infection in periwinkle. This study provides the information necessary to develop detection and management methods for the disease.

Technical Abstract: A novel strain of Lettuce chlorosis virus (LCV) was identified from periwinkle in China (PW) with foliar interveinal chlorosis and plant dwarfing. Complete nucleotide (nt) sequences of genomic RNA1 and RNA2 of the virus are 8,602 nt and 8,456 nt, respectively. The genomic organization of LCV-PW resembles that of a California isolate (LCV-CA), but its RNA2 only encodes 8 open reading frames. The LCV-PW RNA2 is shorter than that of LCV-CA, mainly due to two large deletions. The RNA1 and RNA2 sequences of LCV-PW are 85% and 88% identical to those of LCV-CA, indicating LCV-PW is a novel strain.