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ARS Home » Pacific West Area » Pullman, Washington » Animal Disease Research » Research » Publications at this Location » Publication #339210

Research Project: Development of Detection and Control Strategies for Bovine Babesiosis and Equine Piroplasmosis

Location: Animal Disease Research

Title: Characterization of leukocyte subsets in buffalo (Bubalus bubalis) with cross-reactive monoclonal antibodies specific for bovine MHC class I and class II molecules and leukocyte differentiation molecules

Author
item Davis, William - Washington State University
item Grandoni, Francesco - Agricultural Research Council (CRA)
item Mahmoud, Elnaggar - Washington State University
item Abdellrazeq, Gaber - Washington State University
item Fry, Lindsay
item Mack, Vivtoria - Washington State University
item Marchitelli, Cinzia - Agricultural Research Council (CRA)
item Barile, Vittoria - Agricultural Research Council (CRA)

Submitted to: Developmental and Comparative Immunology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/17/2017
Publication Date: 4/20/2017
Citation: Davis, W.C., Grandoni, F., Mahmoud, E.M., Abdellrazeq, G.S., Fry, L.M., Mack, V., Marchitelli, C., Barile, V.L. 2017. Characterization of leukocyte subsets in buffalo (Bubalus bubalis) with cross-reactive monoclonal antibodies specific for bovine MHC class I and class II molecules and leukocyte differentiation molecules. Developmental and Comparative Immunology. 74:101-109.

Interpretive Summary: New markers to identify immune response cells in buffalo were characterized. This work provides reagents to be used in characterization of immune responses in natural disease and for vaccine development. These particular reagents identify T-lymphocyte and monocyte populations.

Technical Abstract: Although buffalo (Bubalus bubalis) are a major component of the livestock industry worldwide, limited progress has been made in the study of the mechanisms regulating the immune response to pathogens and parasites affecting their health and productivity. This has been, in part, attributable to the limited availability of reagents to study immune responses in buffalo. As reported here, a set of cross reactive monoclonal antibodies (mAbs), developed against bovine, ovine, and caprine leukocyte differentiation molecules (LDM) and major histocompatibility complex (MHC) molecules, were identified and used to compare expression of LDM in Italian and Egyptian buffalo. The results show most of the epitopes identified with the mAbs are conserved on LDM and MHC I and II molecules in both lineages of buffalo. Comparison of the composition of lymphocyte subsets between buffalo and cattle revealed they are similar except for expression of CD2 and CD8 on workshop cluster one (WC1) positive 'd T cells. In cattle, CD8 is expressed on a subset of CD2+/WC1- 'd T cells that are present in low frequency in blood of young and old animals, whereas, CD8-/CD2-/WC1+ 'd T cells are present in high frequency in young animals, decreasing in frequency with age. In the buffalo, CD2 is expressed on a subset of WC1+ 'd T cells and CD8 is expressed on all WC1+ 'd T cells. The availability of this extensive set of mAbs provides opportunities to study the immunopathogenesis of pathogens and parasites affecting the health of buffalo and to study the immunological basis for differences in susceptibility to diseases affecting buffalo and cattle.