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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #339168

Title: Molecular characterization of a novel Luteovirus from peach identified by high-throughput sequencing

item WU, LIPING - Nanchang University
item LIU, HUAWEI - China Agricultural University
item BATEMAN, MARGARITA - Animal And Plant Health Inspection Service (APHIS)
item Liu, Zongrang
item Li, Ruhui

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/12/2017
Publication Date: 5/26/2017
Citation: Wu, L., Liu, H., Bateman, M., Liu, Z., Li, R. 2017. Molecular characterization of a novel Luteovirus from peach identified by high-throughput sequencing. Archives of Virology. 162:2903-2905.

Interpretive Summary: Peaches (Prunus persica) are a globally important stone fruit in the genus Prunus of the rose family. A number of viruses and virus-like pathogens infect peaches, causing various diseases that reduce yields and market value of fruit. In this study, a new virus in peach germplasm undergoing quarantine testing was identified by a sensitive sequencing technology. The complete genomic sequences of two isolates of the virus were determined. Analyses of the genetic information show that the novel virus is in the luteovirus group. The virus was also detected in a peach germplasm collection at a USDA-ARS fruit research facility in West Virginia. The study provides the information necessary to classify the virus, and to help develop detection methods for quarantine and certification programs.

Technical Abstract: Contigs with sequence homologies to Cherry-associated luteovirus were identified by high-throughput sequencing analysis of two peach accessions undergoing quarantine testing. The complete genomic sequences of the two isolates of this virus are 5,819 and 5,814 nucleotides. Their genome organization is typical of luteoviruses, containing eight open reading frames and in a very similar arrangement to other members of the genus. Its genomic sequence is 58-73% identical to those of other Luteovirus species. This is a new virus, for which we suggest the provisional name peach associated luteovirus (PaLV).