|Van Horn, Christopher|
|WU, FENIGIAN - South China Agricultural University|
|ZHENG, ZHENG - South China Agricultural University|
Submitted to: American Phytopathological Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 4/17/2017
Publication Date: 12/1/2017
Citation: Van Horn, C.R., Wu, F., Zheng, Z., Chen, J. 2017. Identification and characterization of plasmids from Xylella fastidiosa using next generation sequencing analyses. American Phytopathological Society Abstracts. S5:81.
Technical Abstract: Xylella fastidiosa causes diseases in many economically important crops, e.g. Pierce’s disease (PD) of grapevines and blueberry bacterial leaf scorch (BBLS) disease in the U.S. Biological research on X. fastidiosa has been difficult due to its nutritional fastidiousness. Genomic research provides a new venue to study Xylella biology. Next generation sequencing (NGS) has recently become a common tool in bacterial biology studies due to the increasing affordability and abundance of data produced. In this study, MiSeq sequencing, an NGS technology, was used to study plasmids in Stag’s leap, a PD strain (X. fastidiosa subsp. fastidiosa) and BB01, a BBLS strain (X. fastidiosa subsp. multiplex). Plasmids are extrachromosomal mobile genetic elements often related to bacterial survival, environmental adaptation, and virulence. Using DNA extracted from in vitro pure culture, MiSeq sequencing generated data sets of 6.59 X 106 sequence reads (301 bp/read) for Stag’s leap, and 1.45 X 107 sequence reads (250 bp/read) for BB01. Analyses of de novo assemblies identified two plasmids: pXFSL01 (20,542 bp) from Stag’s leap carrying a predicted acriflavin resistance gene, and pXFBB01 (31,648 bp) from BB01 carrying a predicted NAD-specific glutamate dehydrogenase gene. The two plasmids likely exist as single copies and are capable of integration into their host chromosomes. These results set forward a base for further studies on Xylella-host interactions.