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ARS Home » Plains Area » College Station, Texas » Southern Plains Agricultural Research Center » Food and Feed Safety Research » Research » Publications at this Location » Publication #338868

Research Project: Ecological Reservoirs and Intervention Strategies to Reduce Foodborne Pathogens in Cattle and Swine

Location: Food and Feed Safety Research

Title: Anti-Salmonella and uric acid-preserving effect of pine bark tannin in composted poultry litter

Author
item Arzola-alvarez, Claudio - Universidad Autonoma De Chihuahua
item Anderson, Robin
item Hume, Michael
item Ruiz-barrera, Oscar - Universidad Autonoma De Chihuahua
item Castillo-castillo, Yamicela - Universidad Autonoma De Ciudad Juarez
item Min, Byeng - Tuskegee University
item Byrd, James - Allen
item Nisbet, David - Dave
item Salinas-chavira, Jaime - University Of Tamaulipas
item Ontiveros-magadan, Marina - Universidad Autonoma De Chihuahua
item Rodriguez-muela, Carlos - Universidad Autonoma De Chihuahua

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/3/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Poultry litter contains appreciable amounts of uric acid which makes it a good crude protein supplement for ruminants, but the litter must be treated to kill bacterial pathogens. Presently, we examined the antimicrobial and uric acid-preserving activity of pine bark tannin during the early stage of poultry litter composting. One year-old wood chip-based poultry litter exposed to 2 to 3 flocks with no use of antibiotics was used in this study. Condensed tannins from pine bark (Pinus palustris) were extracted using a Sephadex LH-20. The litter was distributed (11 g) to 50-mL conical centrifuge tubes (n = 3 tubes/treatment), amended with 1.3 mL of pine bark tannin (9 % tannin wt/vol) or 0.4 M sodium phosphate buffer (control) and then inoculated with a novobiocin and naladixic acid-resistant Salmonella Typhimurium (STNN) to achieve 3.0 log10 CFU/g. The tubes were closed with caps, sealed with parafilm, and incubated at 37 deg C for 3 days after which time the tube contents were serially diluted (10-fold) and plated on 3M E. coli/coliform petri-film and Brilliant Green Agar supplemented with 25 and 20 microg/mL novobiocin and naladixic acid, respectively, for enumeration of wildtype E. coli and the challenge STNN strain. Fluids were analyzed colorimetrically for determination of ammonia, uric acid, and urea concentrations. Analysis of variance revealed that pine bark tannin treatment decreased (P < 0.05) STNN populations in the litter by 0.6 log units compared to controls (6.0 +/- 0.2 log10 CFU/g). Wildtype E. coli populations were unaffected by tannin treatment (6.4 +/- 0.1 log10 CFU/g). Ammonia accumulation was decreased (P < 0.05) 23% in tannin-treated litter compared to the control (2.8 +/- 0.1 micromol/g). Conversely, the residual uric acid concentration was 1.6-fold higher (P < 0.05) in litter treated with the pine bark tannin than in the control litter (15.5 +/- 1.3 micromol/g). Urea concentrations (10.2 +/- 1.6micromol/g) were unaffected by tannin treatment. Results suggest that pine bark tannin treatment may help preserve crude protein contained as uric acid in composted litter while aiding Salmonella control.