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ARS Home » Midwest Area » Urbana, Illinois » Soybean/maize Germplasm, Pathology, and Genetics Research » Research » Publications at this Location » Publication #338688

Research Project: IMPROVED RESISTANCE TO SOYBEAN PATHOGENS AND PESTS

Location: Soybean/maize Germplasm, Pathology, and Genetics Research

Title: Characterization and genetics of multiple soybean aphid biotype resistance in five soybean plant introductions

Author
item Hill, Curtis - University Of Illinois
item Shaio, Derek - University Of Illinois
item Fox, Carolyn - University Of Illinois
item Hartman, Glen

Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/10/2017
Publication Date: 4/4/2017
Citation: Hill, C.B., Shaio, D., Fox, C., Hartman, G.L. 2017. Characterization and genetics of multiple soybean aphid biotype resistance in five soybean plant introductions. Theoretical and Applied Genetics. 130:1335–1348.

Interpretive Summary: Soybean aphid is one of the most important soybean insect pest in the USA. The objectives of this study were to characterize the resistance expressed in five plant introductions (PIs) to four soybean aphid biotypes, determine the mode of resistance inheritance, and identify markers associated with genes controlling resistance in these accessions. Five soybean PIs, from an initial set of 3000 PIs, were tested for resistance against soybean aphid biotypes 1, 2, 3, and 4 in choice and no-choice tests. Of these five PIs, PI 587663, PI 587677, and PI 587685 were resistant to all four biotypes, while PI 587972 and PI 594592 were resistant to biotypes 1, 2, and 3. The five soybean plant introductions expressed resistance to multiple soybean aphid biotypes with two introductions having resistance genes located in the Rag1, Rag2, and Rag3 regions, two introductions having resistance genes located in the Rag1 and Rag2 regions, and one introduction having a resistance gene located in the Rag2 region. This information is useful to scientists interested in insect resistance and the information will be used by breeders in industry to develop more resistant soybean cultivars for soybean growers.

Technical Abstract: Soybean aphid (Aphis glycines Matsumura) is the most important soybean [Glycine max (L.) Merr.] insect pest in the USA. The objectives of this study were to characterize the resistance expressed in the five plant introductions (PIs) to four soybean aphid biotypes, determine the mode of resistance inheritance, and identify markers associated with genes controlling resistance in these accessions. Five soybean PIs, from an initial set of 3000 PIs, were tested for resistance against soybean aphid biotypes 1, 2, 3, and 4 in choice and no-choice tests. Of these five PIs, PI 587663, PI 587677, and PI 587685 expressed antibiosis against all four biotypes, while PI 587972 and PI 594592 expressed antibiosis against biotypes 1, 2, and 3. F2 populations derived from PI 587663 and PI 587972 were evaluated for resistance against soybean aphid biotype 1, and populations derived from PIs 587677, 587685, and 594592 were tested against biotype 3. In addition, F2:3 plants were tested against biotypes 2 and 3. Genomic DNA from F2 plants was screened with markers linked to Rag1, Rag2, Rag3, and rag4 soybean aphid resistance genes. Results indicated that PI 587663 and PI 594592 had three resistance genes located in the Rag1, Rag2, and Rag3 regions, PI 587677 and PI 587685 had two resistance genes located in the Rag1 and Rag2 regions, and PI 587972 had one resistance gene located in the Rag2 region. The multi-biotype aphid resistance and information on resistance gene markers will be useful to improve soybean aphid resistance in soybean.