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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #338273

Research Project: CHARACTERIZING, DETECTING, AND ELIMINATING PATHOGENS TO ENABLE THE SAFE INTRODUCTION OF PLANT GENETIC RESOURCES

Location: National Germplasm Resources Laboratory

Title: Genome characterization and genetic diversity of sweet potato symptomless virus 1: a mastrevirus with an unusual nonanucleotide

Author
item Cao, Mengji - Southwest University
item Lan, Pingxiu - Yunnan Agricultural University
item Li, Fan - Yunnan Agricultural University
item Abad, Jorge - Animal And Plant Health Inspection Service (APHIS)
item Zhou, Changyong - Southwest University
item Li, Ruhui

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/27/2017
Publication Date: 5/11/2017
Citation: Cao, M., Lan, P., Li, F., Abad, J., Zhou, C., Li, R. 2017. Genome characterization and genetic diversity of sweet potato symptomless virus 1: a mastrevirus with an unusual nonanucleotide. Archives of Virology. 162:2881-2884.

Interpretive Summary: Sweet potato [Ipomoea batatas (L.) Lam] is an important root/tuber crop worldwide, especially in Africa and Asia. The crop is susceptible to many viruses including Sweet potato symptomless virus 1 (SPSMV-1), and mixed infections frequently cause serious diseases that impact sweet potato production. In this study, SPSMV-1 was identified by next generation sequencing and the complete genomic sequences of nine SPSMV-1 isolates were determined. Analyses show that SPSMV-1 is distinct from other viruses in its family and genus. A PCR detection method was developed and used to test for SPSMV-1 in sweet potato production fields in China. The results suggest the virus is widely distributed in China. This study provides the information necessary for proper classification of SPSMV-1. The detection method helps to develop better testing and management strategies for this sweet potato virus.

Technical Abstract: Complete genomic sequences of nine isolates of sweet potato symptomless virus 1 (SPSMV-1), a virus of genus Mastrevirus in the family Geminiviridae, was determined to be 2,559-2,602 nucleotides from sweet potato accessions from different countries. These isolates shared genomic sequence identities of 97-100%. The SPSMV-1 genome has an unusual nonanucleotide (TAAGATTCC) in large intergenic region and contains an additional open reading frame C3, which is conserved in all docot-infecting mastreviruses. Ten smaller-than-genome and a larger-than-genome DNAs were also obtained from infected plants. SPSMV-1 was detected in 51.4% of sweet potato field samples from China, suggesting the virus is widely distributed in China.