|GREEN, KELSIE - University Of Idaho
|QUITO-AVILA, DIEGO - Centro De Investigaciones Biotecnologicas Del Ecuador
|KARASEV, ALEX - University Of Idaho
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 3/26/2017
Publication Date: 12/1/2017
Citation: Green, K., Mollov, D.S., Quito-Avila, D., Karasev, A. 2017. Characterization of a new tymovirus from Ecuador. Phytopathology. S5:97.
Technical Abstract: Naranjilla (Solanum quitoense) is a solanaceous shrub cultivated in Ecuador for fresh fruit and juice production. A naranjilla plant exhibiting stunting and chlorotic spots was sampled near Quito, Ecuador. An infectious agent was transferred to indicator plants by mechanical inoculation, and subsequently characterized at biological and molecular levels. The pathogen from naranjilla systemically infected eggplant producing mild mosaic symptoms, and naranjilla producing chlorotic spots, mosaic, and stunting. However, it did not infect potato or tobacco. An RNA-Seq analysis conducted on inoculated symptomatic Nicotiana benthamiana plants produced a single sequence contig sharing the closest relationship with several tymoviruses. The entire genome of the virus was amplified using RT-PCR and sequenced by the Sanger methodology. The genome was found to encode a set of open reading frames typical of tymoviruses, with the highest nucleotide sequence identity to the closest tymovirus Eggplant mosaic virus, at below 70%. The data suggest that this is a new tymovirus infecting naranjilla, for which the tentative name Naranjilla chlorotic mosaic virus (NarCMV) is proposed.