|WOODS, AUTUMN - Alabama A & M University|
|JACKSON-DAVIS, ARMITRA - Alabama A & M University|
|CEBERT, ERNST - Alabama A & M University|
|Hinton, Jr, Arthur|
|KASSAMA, LAMIN - Alabama A & M University|
Submitted to: International Association for Food Protection Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/18/2017
Publication Date: 7/9/2017
Citation: Woods, A., Jackson-Davis, A., Cebert, E., Hinton Jr, A., Kassama, L. 2017. Efficacy of jatropha curcas plant extract against the survival of salmonella enteritidis[abstract]. International Association for Food Protection Proceedings. PI:193.
Interpretive Summary: none
Technical Abstract: Introduction: The use of plant-derived antimicrobials has shown to be effective at inhibiting microbial growth. Although Jatropha curcas is known to possess antimicrobial properties, its efficacy against Salmonella Enteritidis has not yet been investigated. Purpose: The purpose of this study was to evaluate the effectiveness of various concentrations of Jatropha curcas plant extracts against S. Enteritidis in a broth system. Methods: The efficacy of various concentrations of J. curcas (10 mg/mL, 12.5 mg/mL, 25 mg/mL, 50 mg/mL, 100 mg/mL, and 60 mg/mL for root extracts) extracted using different solvents (methanol and water) against the survival of S. Enteritidis was determined. Plants were separated into stem, leaf, and root parts, freeze-dried. Extracts were prepared by soaking 5 g of the ground plant material (stem, leaf, and root) in 50 ml of each solvent. The antimicrobial abilities of the extracts were then determined using a Bioscreen C Turbidometer at 600 nm and 35°C for 24 hours. The control consisted of S. Enteritidis without the presence of any extract. Results: The results of the Bioscreen analysis indicate that J. curcas does have some antimicrobial abilities against S. Enteritidis. ANOVA analysis revealed a significant difference between the methanol and water based stem and root extracts at each of the various concentrations (p < 0.05). According to Fishers LSD test, only leaf/water extract at 50 mg/ml was significantly different (p < 0.05) from all other leaf extracts (water and methanol). Observed optical density values (average = 0.507) demonstrated that the various concentrations of extracts were lower than that of the control (1.229). Significance: The ability of J. curcas to inhibit the growth of Salmonella Enteritidis indicates that these extracts could be used during post-harvest cleaning and sanitizing operations by incorporating the use of this plant derived antimicrobial as a sanitizer.