|Crouse, Matthew - North Dakota State University|
|Ward, Alison - North Dakota State University|
|Cushman, Robert - Bob|
|Mclean, Kyle - North Dakota State University|
|Dahlen, Carl - North Dakota State University|
|Borowicz, Pawel - North Dakota State University|
|Reynolds, Lawrence - North Dakota State University|
|Caton, Joel - North Dakota State University|
Submitted to: Journal of Trace Elements in Medicine and Biology
Publication Type: Abstract Only
Publication Acceptance Date: 4/10/2017
Publication Date: 5/10/2017
Citation: Crouse, M.S., Ward, A.K., Cushman, R.A., McLean, K.J., Dahlen, C.R., Borowicz, P.P., Reynolds, L.P., Caton, J.S. 2017. Maternal nutrition during the first 50 d of gestation alters expression of metal-binding genes in fetal cerebrum [abstract]. Journal of Trace Elements in Medicine and Biology. 41S:63. http://doi.org/10.1016/j.temb.2017.03.261.
Technical Abstract: We hypothesized that maternal nutrition during the first 50 d of gestation would alter the metal-binding transcriptome of the developing cerebrum. 14 beef heifers were estrus synchronized and assigned to 2 treatments at breeding (CON-100% of requirements; RES-60% of CON). Heifers were ovariohysterectomized on d 50 of gestation and fetal cerebra were dissected, flash frozen, RNA extracted, and RNA-Sequencing conducted. Transcriptome analysis was run via the Tuxedo Suite in collaboration with USDA-ARS-MARC and gene clusters were examined with DAVID 6.8. 151 genes were utilized for cluster analysis (P<0.01; 29 q<0.10). The metal-binding cluster contained 24 genes (Enrichment Score 1.01; P=0.006; Benjamini=0.047), 4-Fe, 10-Zn, 2-Cu, and 8 for other metal binding genes. 23 metal-binding genes were upregulated in RES (Min=2.26, Max=46.29; Avg=8.28-fold greater), and 1 in CON (3.51-fold greater). Thus, maternal nutrition during the first 50 days of gestation impacted expression of metal-binding genes in bovine fetal cerebrum.