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ARS Home » Midwest Area » East Lansing, Michigan » Sugarbeet and Bean Research » Research » Publications at this Location » Publication #337430

Research Project: Genetic Dissection of Traits for Sugar Beet Improvement

Location: Sugarbeet and Bean Research

Title: Organization of a resistance gene cluster linked to rhizomania resistance in sugar beet

item FUNK, ANDY - Michigan State University
item STEVANATO, PIERGIORGIO - University Of Padua
item TAGUCHI, KAZUNORI - Hokkaido Research Organization
item McGrath, Jon

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/12/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Genetic resistance to rhizomania has been in use for over 40 years. Characterization of the molecular basis for susceptibility and resistance has proved challenging. Nucleotide-binding leucine-rich-repeat-containing (NB-LRR) genes have been implicated in numerous gene-for-gene resistance interactions, including resistance to rhizomania due to the Rz2 gene. One hypothesis is that Rz1 is also an NB-LRR gene. To pursue this avenue further, additional nucleotide-binding (NB) sequences were identified in 16 MB of Chromosome 3 encompassing the Rz1 and Rz2 loci. These NB-like sequences were identified using a hidden Markov model derived from resistance gene NB domains and provided tentative locations for twenty NB-LRR loci. These sequences exhibit spatial clustering correlated with their phylogenetic clustering, which suggests evolution through gene duplication. Comparing two populations sharing a common parent we describe variation at these 20 NB-ARC sequences. This includes the presence of segregating repetitive elements in or adjacent to 16 of the 20 predicted NB-LRR sequences. Transcript evidence is given for the expression of eight of these NB loci in root tissue of the common parent C869, with trace transcript evidence for an additional seven (15 putatively expressed loci in total in C869).