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ARS Home » Southeast Area » Mayaguez, Puerto Rico » Tropical Crops and Germplasm Research » Research » Publications at this Location » Publication #337218

Title: Genomic dissection of anthracnose resistant response in sorghum [Sorghum bicolor (L.)]

item Cuevas, Hugo
item Prom, Louis
item Knoll, Joseph - Joe
item VERMERRIS, WILFRED - University Of Florida

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/15/2017
Publication Date: N/A
Citation: N/A

Interpretive Summary: Anthracnose (Colletotrichum sublineolum) is one of the most destructive diseases of sorghum (Sorghum bicolor L. Moench) affecting all aerial tissues of the plant. The identification of resistance genes is the most effective and safe strategy for disease control. In this study, anthracnose resistance present in the line SC112-14, QL3 and IS18760 were evaluated in Texas, Georgia and Florida. The results showed that disease resistance present in SC112-14 is effective in all locations, while QL3 and IS18760 are effective only in Texas. The resistance locus in SC112-14 was located at the distal region of chromosome 5, and validated by greenhouse evaluation against multiple pathotypes.

Technical Abstract: The goal of this project is to use a genomics-based approaches to identify anthracnose resistance loci from diverse sorghum germplasm as an effort to the disease resistance mechanism of at least one of these genes. This information will provide plant breeders with a tool kit that can be used to maximize disease resistance for sorghum production areas. We developed three sets of recombinant inbred lines (RILs) derived from the cross of three resistant sources (SC112-14, QL3 and IS18760) and a commonly high susceptible line PI609251. A high density linkage map was constructed for the RIL population derived from the cross of SC112-14 x PI609251 using 1,671 single nucleotide polymorphism sites (SNPs). We later used this map to select a reduced sample of individuals with a high number of recombinant breakpoints which were evaluated under greenhouse conditions against 10 pathotypes from Texas, Georgia, Arkansas, and Puerto Rico. In parallel, the three RILs were evaluated under field conditions at Gainesville, FL, Tifton, GA, and College Station, TX. In addition, we identified two QTL’s associated with the resistant response in sorghum line Bk7 (chromosome 7 and 9; Felderhoff et al. 2016, G3), which are now being analyzed in more detail to identify the underlying resistance genes. Anthracnose resistance responses were observed for the RILs derived from SC112-14 at Gainesville, FL, Tifton, GA, and College Station, TX. However, the RILs derived from QL3 and IS18760 showed a resistance response only against pathotypes from College Station, TX. The linkage mapping analysis of SC112-14 identified one locus at distal region of chromosome 5 which is controlling the resistance response against pathotypes from the three locations (Fig. 1A). Remarkably, some RILs showed variable resistant response across locations indicating this locus may be constituted by a cluster of multiple resistance genes. Greenhouse evaluation against multiple particular pathotypes validated this locus and delimited its location to a 250 Kb genomic region (Fig. 1B). The observed difference among greenhouse and field evaluations indicated that both analysis are complementary and necessary to dissect the anthracnose resistant locus. We also determined that resistant locus of in chromosome 9 present in sorghum line Bk7 enclosed 12 candidate genes that were validated to be expressed in leaves. We will be evaluating a larger number of segregating progenies to reduce this genomic region to candidate genes for functional analysis. Simultaneously, we are constructing high density genetic maps for RILs derived from QL3 and IS18760 in order to map and study their resistance. The 12 candidate genes from Bk7 inheritance study are now being targeted for down-regulation using virus-induced gene silencing.