Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 11/1/2017
Publication Date: 2/12/2018
Citation: Lippolis, J.D., Nally, J.E. 2018. Considerations for farm animal proteomics experiments: An introductory view gel based versus non-gel based approaches. In: de Almeida A., Eckersall D., Miller I., editors. Proteomics in Domestic Animals: from Farm to System Biology. Basel, Switzerland: Springer International Publishing AG. p. 7-16. https://doi.org/10.1007/978-3-319-69682-9_2.
Technical Abstract: This chapter gives an outline for the preparation and completing proteomic experiments. We discuss the importance of sample preparation, sample separation, and protein detection and identification. We also compare and contrast the gel-based protein separation with non gel-based separation techniques. The type of proteomics research question will often determine the type of protein separation suited for the experiment. In addition, the choice between gel and gel-free protein separation in a proteomic experiment may simply be a matter of preference, convenience, or the possession of specific equipment or mass spectrometer. One laboratory may possess and have the necessary skills with HPLC equipment, whereas, another laboratory may prefer working with 2-D gel equipment. Each type of protein separation can be used independently or in combination. In most cases, some sort of protein or peptide separation is necessary to achieve the goals of a proteomics experiment. Careful consideration of the strengths of each type of separation and how they align with project goals prior to the experiment will help to ensure a successful outcome.