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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #336515

Research Project: Integrated Research Approaches for Improving Production Efficiency in Salmonids

Location: Cool and Cold Water Aquaculture Research

Title: Transcriptome response to hormonal manipulation of follicle-enclosed oocytes in rainbow trout

item Ma, Hao
item Gao, Guangtu
item Weber, Gregory - Greg

Submitted to: Plant and Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 12/20/2016
Publication Date: 4/14/2017
Citation: Ma, H., Gao, G., Weber, G.M. 2017. Transcriptome response to hormonal manipulation of follicle-enclosed oocytes in rainbow trout. [abstract] Plant and Animal Genome Conference. P1005.

Interpretive Summary:

Technical Abstract: Captive fish often display reproductive dysfunction associated with follicle maturation. Gonadotropins and the progestogen maturation-inducing hormones (MIH) are important regulators of follicle maturation; however, their actions including regulating follicle maturation are not fully understood. The investigation of the transcriptome response to treatment with these hormones was conducted to further elucidate the actions of these regulators. Follicle enclosed oocytes from rainbow trout competent to undergo the resumption of meiosis in response to the MIH were incubated in vitro for 24hrs with or without either MIH or salmon pituitary hormone extract (SPH). A total of 1,691, 4,239 and 691 differentially expressed transcripts (DETs) were identified from our transcriptome sequencing datasets for control vs freshly excised tissue, control vs MIH, and control vs SPH treatment comparisons respectively. There are 117 DETs shared by the three comparisons, of which 48 were associated with significantly enriched GO terms, mainly, metabolic process, inflammatory response, signal transduction, and transcription regulation. A total of 356 DETs were specifically shared by both MIH and SPH treatments, among which 318 DETs demonstrated similar responses with each hormone. The responses of selected genes to hormone treatment were further confirmed by RT-qPCR analysis. The global changes in gene expression profiling revealed both disparate and overlapping actions of the hormone treatments which further elucidate the regulation of processes leading to the production of viable gametes.