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ARS Home » Southeast Area » Oxford, Mississippi » Natural Products Utilization Research » Research » Publications at this Location » Publication #336155
Research Project: Discovery and Development of Natural Products for Pharmaceutical and Agrochemical Applications II

Location: Natural Products Utilization Research

Title: Prenylated flavonol glycosides Epimedium grandiflorum: Cytotoxicity and evaluation against inflammation and metabolic disorders

Author
item ZULFIQAR, FAZILA - University Of Mississippi
item KHAN, SHABANA - University Of Mississippi
item ROSS, SAMIR - University Of Mississippi
item ALI, ZULFIQAR - University Of Mississippi
item KHAN, IKLAS - University Of Mississippi

Submitted to: Phytochemistry Letters
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/22/2017
Publication Date: 4/27/2017
Citation: Zulfiqar, F., Khan, S.I., Ross, S.A., Ali, Z., Khan, I.A. 2017. Prenylated flavonol glycosides Epimedium grandiflorum: Cytotoxicity and evaluation against inflammation and metabolic disorders. Phytochemistry Letters. 20:160-167.

Interpretive Summary: Two new prenylated flavonol glycosides, and 28 previously known compounds were isolated from the methanol extract of Epimedium grandiflorum. Structure elucidation was achieved by means of spectroscopic and spectrometric techniques including 1D and 2D NMR and HRESIMS. The methanol extract and the isolated compounds were evaluated for their activity towards several targets related to inflammation and metabolic disorder including NF-kB, iNOS, PPARa and PPAR'. Moreover, their cytotoxic activity against four cancer cell lines and two noncancerous kidney cell lines was also evaluated.

Technical Abstract: Two new prenylated flavonol glycosides, epimedigrandiosides A and B (1 and 2), and 28 previously known compounds including prenylated flavonol derivatives, flavonol glycoside, megastigmanes, phenyl alkanoids, sesquiterpenoid glycoside, lignan, and hexene glucoside were isolated from the methanol extract of Epimedium grandiflorum. Structure elucidation was achieved by means of spectroscopic and spectrometric techniques including 1D and 2D NMR and HRESIMS. The absolute configuration of sugars was determined by chemical methods. Structure elucidation of 3"'-carbonyl-2"-L-quinovosyl icariin (19) was not previously described, so its 1H and 13C NMR data were reported for the first time. The methanol extract and the isolated compounds were evaluated for their activity towards several targets related to inflammation and metabolic disorder including NF-kB, iNOS, PPARo. and PPARy. Moreover, their cytotoxic activity against four cancer cell lines (SK-MEL, KB, BT-549, SK-OV-3) and two noncancerous kidney cell lines (LLC-PK1 and Vero) was also evaluated.