Location: Healthy Processed Foods ResearchTitle: Mice fed high-fat diets supplemented with peels of three apple varieties, Red delicious, Golden delicious, or Black Arkansas, have reduced weight and differences in gut microbiota (abstract)
|ELKAHOUI, SALEM - Centre De Biotechnologie De Borj Cédria|
|Yokoyama, Wallace - Wally|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/2/2017
Publication Date: 4/5/2017
Citation: Friedman, M., Elkahoui, S., Sturges, D.G., Bartley, G.E., Levin, C.E., Yokoyama, W.H. 2017. Mice fed high-fat diets supplemented with peels of three apple varieties, Red delicious, Golden delicious, or Black Arkansas, have reduced weight and differences in gut microbiota (abstract). Meeting Abstract. 2017. Presented at the 253rd American Chemical Society National Meeting & Exposition, San Francisco.
Technical Abstract: The present study investigated the potential of apple peels, known to be high in polyphenols, to mitigate the deleterious effects of a high-fat diet in mice. Apple peels from three distinct varieties, Red delicious, Golden delicious, and Black Arkansas, were prepared by hand-peeling fresh apples and then freeze-drying and grinding the peels into powders. Mice diets, (25% by weight fat) were supplemented with either 10% or 20% apple peels for three weeks. A positive control group was fed a 1% or 2% commercial apple phenolic extracts. Total phenolic content and flavonoid profiles by HPLC were determined in the peels and in feces. There was a dose response in weight gain, adipose weight, liver weight, and blood lipids. Expression of stearoyl-CoA desaturase (SCD-1) and cytochrome P450, family 51 (Cyp51) were related to phenolic content, and expression of SCD-1 was correlated with adipose weight. The microbiota of feces from mice fed Golden and Red delicious peels, and the phenolic extracts were analyzed by next-generation sequencing (NGS). The results showed differences associated with apple peel source. Apple peels have the potential to serve as an antiobesity functional food additive.