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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #335598
Title: Divergence in substrate specificity by the vOTU domain of various strains of highly-pathogenic PRRSV and the implications to pathogenicity

Author
item CHENGE, JUDE - University Of Georgia
item BESTER, STEPHANIE - University Of Georgia
item RYLL, ALEXANDRA - University Of Georgia
item Faaberg, Kay
item PEGAN, SCOTT - University Of Georgia

Submitted to: European Society for Veterinary Virology
Publication Type: Abstract Only
Publication Acceptance Date: 10/10/2016
Publication Date: 10/19/2016
Citation: Chenge, J., Bester, S.M., Ryll, A., Faaberg, K.S., Pegan, S.D. 2016. Divergence in substrate specificity by the vOTU domain of various strains of highly-pathogenic PRRSV and the implications to pathogenicity. 6th European Congress of Virology. Paper No. P1-34.

Interpretive Summary:

Technical Abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) is widespread with a high variation in sequence and virulence among the divergent strains and causes an economically destructive disease. A viral ovarian domain protease (vOTU) has been previously identified within the nonstructural protein nsp2 of PRRSVs. As with many vOTU family proteases, these proteases have been suggested to be a virulence factor for PRRSV through their ability to potentially cleave ubiquitin and ISG15 conjugated substrates. Recently, significantly divergence in substrate specificity between two vOTUs from PRRSV strains varying in virulence had been revealed. Here we examine the substrate differences of four PRSSV strains, the previously characterized JXwn06 and MLV strains along with strains SDSU73 and NADC31. This additional information provides a more complete view of the range PRRSV vOTU possess in substrate specificity and suggests potential correlation between substrate specificity of strains and differences in virulence.