|Bauermann, Fernando - South Dakota State University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/14/2016
Publication Date: N/A
Technical Abstract: Bovine viral diarrhea virus (BVDV) is an RNA virus that causes respiratory disease in cattle. MicroRNAs have been proposed as indicators of exposure to respiratory pathogens. However, microRNA profiles in cattle exposed to BVDV are currently nonexistent and few studies have been reported; therefore, the objective of this study was to identify microRNAs in cattle that were challenged with a non-cytopathic field strain of BVDV. Five colostrum deprived neonate Holstein calves were challenged with BVDV (treatment) and 4 were mock challenged (control). Serum from all calves was collected at 4 time points: (1) baseline, collected prior to challenge; (2) acute response, collected 4 days post challenge; (3) recovery response, collected 9 days post challenge; and (4) post response, collected 16 days post challenge. Due to limiting animal space in a biosafety level 2 facility, the experiment consisted of two phases. RNA was extracted from sera, and small non-coding RNAs were obtained using next-generation sequencing. A total of 905,861 sequences were identified as microRNAs. Bta-miR-423-5p was identified (P = 0.007) as being differentially expressed between challenged and control animals across time points. In treatment animals, bta-miR-423-5p peaked from baseline levels during acute response then steadily declined, while the number of sequences among control animals steadily declined until after recovery response. Other studies have identified the differential expression of bta-miR-423-5p between cattle challenged with mycoplasma and control animals. Further studies are needed to establish if bta-miR-423-5p could potentially be used as a biomarker for exposure to BVDV.