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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Plant Polymer Research » Research » Publications at this Location » Publication #335225

Research Project: Conversion of Polysaccharides and Other Bio-based Materials to High-Value, Commercial Products

Location: Plant Polymer Research

Title: Amperometric biosensor for Salmonella typhimurium detection in milk

item ALEXANDRE, D - Universidade Estadual Do Ceara
item MELO, A - Universidade Estadual Do Ceara
item BORGES, M - Embrapa
item FIGUEIREDO, E - Federal University - Brazil
item Biswas, Atanu
item Cheng, Huai
item ALVES, C - Universidade Estadual Do Ceara

Submitted to: Symposium Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 10/20/2016
Publication Date: 10/27/2016
Citation: Alexandre, D.L., Melo, A., Furtado, R.F., Borges, M.F., Figueiredo, E., Biswas, A., Cheng, H.N., Alves, C.R. 2016. Amperometric biosensor for Salmonella typhimurium detection in milk. Proceedings of the 25th Food Science and Technology Brazilian Congress (CBCTA) and the 10th CIGR Section IV International Technical Symposium, October 24-27, 2016, Gramado/RS, Brazil.

Interpretive Summary: Salmonella in food can cause sickness, and its rapid and accurate detection is important to ensure food safety. In this work we have developed an immunosensor for diagnosing Salmonella contamination in milk. This method will help milk producers to rapidly detect Salmonella-contaminated milk. The method may be generalized to other food items and will be useful to the food industry in general. This work is also a timely addition to the food safety knowledge base.

Technical Abstract: This paper reports an amperometric biosensor for rapid and sensitive Salmonella Typhimurium detection in milk. The biosensor was assembled from the self-assembled monolayers technique on a gold surface. In this device, polyclonal antibodies were oriented by protein A. The biosensor structure was characterized by cyclic voltammetry and the analytical response was obtained by a chronoamperometry technique using a sandwich system labeled with peroxidase enzyme. The performance of the biosensor was determined and the device was tested in skim and whole milk. The response curve of the biosensor showed a low limit of detection of 10 UFC mL-1 and detection time of 125 min. The results of the biosensor performance in milk attested that the device was able to detect S. Tiphymurium quickly in a simple laboratory setting and without enrichment step.