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ARS Home » Midwest Area » St. Paul, Minnesota » Cereal Disease Lab » Research » Publications at this Location » Publication #334436

Research Project: CEREAL RUST FUNGI: GENETICS, POPULATION BIOLOGY, AND HOST-PATHOGEN INTERACTIONS

Location: Cereal Disease Lab

Title: Dissecting plant chromosomes by the use of ionizing radiation

Author
item KIANIAN, PENNY - University Of Minnesota
item Liberatore, Katie
item MILLER, MARISA - University Of Minnesota
item HEGSTAD, JUSTIN - North Dakota State University
item Kianian, Shahryar

Submitted to: Book Chapter
Publication Type: Book / Chapter
Publication Acceptance Date: 4/15/2016
Publication Date: 8/1/2016
Citation: Kianian, P., Liberatore, K.L., Miller, M., Hegstad, J., Kianian, S. 2016. Dissecting plant chromosomes by the use of ionizing radiation. In: Kianian, S.K., Kianian, P.M.A. Methods in Molecular Biology. New York, NY: Springer Publishing. p. 91-102.

Interpretive Summary: Radiation treatment of genomes is used to generate chromosome breaks for numerous applications. This protocol describes the preparation of seeds and the determination of the optimal level of irradiation dosage for the creation of a radiation hybrid (RH) population. These RH lines can be used to generate high resolution physical maps for the assembly of sequenced genomes as well as the fine mapping of genes. This procedure can also be used for mutation breeding and forward/reverse genetics.

Technical Abstract: Radiation treatment of genomes is used to generate chromosome breaks for numerous applications. This protocol describes the preparation of seeds and the determination of the optimal level of irradiation dosage for the creation of a radiation hybrid (RH) population. These RH lines can be used to generate high resolution physical maps for the assembly of sequenced genomes as well as the fine mapping of genes. This procedure can also be used for mutation breeding and forward/reverse genetics.