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ARS Home » Northeast Area » Beltsville, Maryland (BARC) » Beltsville Agricultural Research Center » National Germplasm Resources Laboratory » Research » Publications at this Location » Publication #333882

Research Project: CHARACTERIZING, DETECTING, AND ELIMINATING PATHOGENS TO ENABLE THE SAFE INTRODUCTION OF PLANT GENETIC RESOURCES

Location: National Germplasm Resources Laboratory

Title: Complete nucleotide sequence of Clematis chlorotic mottle virus, a new member of the family Tombusviridae

Author
item Mclaughlin, Margaret
item Lockhart, Ben - University Of Minnesota
item Jordan, Ramon
item Denton, Geoff - Royal Horticultural Society
item Mollov, Dimitre

Submitted to: Archives of Virology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/4/2017
Publication Date: 4/1/2017
Citation: Mclaughlin, M.S., Lockhart, B., Jordan, R.L., Denton, G., Mollov, D.S. 2017. Complete nucleotide sequence of Clematis chlorotic mottle virus, a new member of the family Tombusviridae. Archives of Virology. 162(5):1373-1379.

Interpretive Summary: Clematis is a woody climbing vine with attractive flowers widely used for ornamental purposes. Over 100 clematis plants (more than 20 varieties) from several U.S. states and the U.K. were observed with virus-like symptoms. Spherical virus particles were found in symptomatic plants using transmission electron microscopy. The virus was named Clematis chlorotic mottle virus (ClCMV) and its complete genomic sequence was determined. Based on phylogenetic analysis and genomic sequence comparisons to known viruses, ClCMV was characterized as a new member of the genus Pelarspovirus, in the family Tombusviridae. Completing the ClCMV genome sequence facilitates developing reliable detection methods and better control measures for this newly described virus of Clematis. This information will be useful to plant diagnosticians and regulatory agencies, as well as the ornamental and the landscape industries.

Technical Abstract: Clematis chlorotic mottle virus (ClCMV) is a previously undescribed virus associated with yellow mottling and veining, chlorotic ring spots, line pattern mosaics, and flower distortion and discoloration on ornamental Clematis. The ClCMV genome is 3,880nt in length with 5 putative open reading frames (ORFs) encoding a 26-kDa protein (ORF 1), an 86-kDa replicase protein (ORF 2), two centrally located movement proteins (ORF 3 and 4), and a 37-kDa capsid protein (ORF 5). Based on morphological, genomic, and phylogenetic analysis, ClCMV is predicted to be a member of the genus Pelarspovirus in the family Tombusviridae.