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ARS Home » Southeast Area » Tifton, Georgia » Crop Protection and Management Research » Research » Publications at this Location » Publication #333446

Research Project: Developing Genomic Approaches to Improve Resistance to Diseases and Aflatoxin Contamination in Peanut and Corn

Location: Crop Protection and Management Research

Title: Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress

Author
item Katama, Ramesh - FLORIDA A & M UNIVERSITY
item Sakata, Katsumi - MAEBASHI INSTITUTE OF TECHNOLOGY
item Suravajhala, Prashanth - BIOCLUES
item Pechand, Tibor - MISSISSIPPI STATE UNIVERSITY
item Kambiranda, Devaiah - FLORIDA A & M UNIVERSITY
item Naik, Karamthot - ACHARYA NG RANGA AGRICULTURAL UNIVERSITY (ANGRAU)
item Guo, Baozhu
item Basha, Sheikh - FLORIDA A & M UNIVERSITY

Submitted to: Journal of Proteomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/28/2016
Publication Date: 6/6/2016
Citation: Katama, R., Sakata, K., Suravajhala, P., Pechand, T., Kambiranda, D., Naik, K., Guo, B., Basha, S. 2016. Comparative leaf proteomics of drought-tolerant and -susceptible peanut in response to water stress. Journal of Proteomics. 143:209-226. doi:10.1016/j.jprot.2016.05.031.

Interpretive Summary: Peanuts are a major source of plant protein grown in subtropical and tropical regions of the world. Pre-harvest aflatoxin contamination is a major problem that affects peanut crop yield and food safety. Drought increases invasions of the aflatoxin producing fungus and thus affects the quality and yield of peanut. Therefore, more studies involving the effects of drought stress to determine the molecular changes will enhance our understanding of the key metabolic pathways involved in the combined stresses. In this study, we have identified water stress responsive proteins of two contrast cultivars for drought tolerance to unravel the underlying mechanisms involved in their differential response to water stresses. Vemana is known to be highly drought-tolerant and has an excellent yield, while Florunner is a popular cultivar despite its susceptibility to drought. This study reveals the identity and functions of low and high abundant proteins, and newly induced leaf proteins in drought tolerant and sensitive cultivars due to water stress, and their possible roles in tolerance mechanism were investigated. Protein interaction prediction analysis suggests that more proteins interacting in tolerant cultivar were shown to activate other proteins in directed system response networks.

Technical Abstract: Water stress (WS) predisposes peanut plants to fungal infection resulting in pre-harvest aflatoxin contamination. Major changes during water stress including oxidative stress, lead to destruction of photosynthetic apparatus and other macromolecules within cells. Two peanut cultivars with diverse drought tolerance characteristics were subjected to WS, and their leaf proteome was compared using two-dimensional electrophoresis complemented with MALDI-TOF/TOF mass spectrometry. Ninety-six protein spots were differentially abundant to water stress in both cultivars that corresponded to 60 non-redundant proteins. Protein interaction prediction analysis suggests that 42 unique proteins showed interactions in tolerant cultivar while 20 showed interactions in the susceptible cultivar, activating other proteins in directed system response networks. Four proteins: glutamine ammonia ligase, chitin class II, actin isoform B, and beta tubulin, involved in metabolism, defense and cellular biogenesis, are unique in tolerant cultivar and showed positive interactions with other proteins. In addition, four proteins: serine/threonine protein phosphate PP1, choline monooxygenase, peroxidase 43, and SNF1-related protein kinase regulatory subunit beta-2, that play a role as cryoprotectants through signal transduction, were induced in drought tolerant cultivar following WS. Eleven interologs of these proteins were found in Arabidopsis interacting with several proteins and it is believed that similar mechanisms/pathways exist in peanut.