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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #333232

Research Project: Integrated Research Approaches for Improving Production Efficiency in Salmonids

Location: Cool and Cold Water Aquaculture Research

Title: Estradiol regulates expression of miRNAs associated with myogenesis in rainbow trout

item KOGANTI, PRASANTHI - West Virginia University
item WANG, JIAN - West Virginia University
item Cleveland, Beth
item MA, HAO - West Virginia University
item Weber, Gregory - Greg
item YAO, JIANBO - West Virginia University

Submitted to: Molecular and Cellular Endocrinology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/13/2016
Publication Date: 3/5/2016
Citation: Koganti, P., Wang, J., Cleveland, B.M., Ma, H., Weber, G.M., Yao, J. 2016. Estradiol regulates expression of miRNAs associated with myogenesis in rainbow trout. Molecular and Cellular Endocrinology. 443:1-14. doi:10.1016/j.mce.2016.12.014.

Interpretive Summary: 17-Estradiol is an estrogenic hormone that reduces rainbow trout growth and muscle mass, although the mechanisms through which estradiol exerts these effects is not fully understood. This study investigated the ability of estradiol to regulate micro-RNAs (miRNA) in muscle as a potential mechanism involved in the response. miRNAs are small nucleotides molecules that regulate expression of other genes and they are being increasingly recognized for their physiological significance. Thirty-six miRNAs were found to be regulated in muscle of rainbow trout exposed to estradiol. Several of these miRNAs have the ability to regulate genes directly involved in muscle breakdown and cell cycling. These findings indicate that the estradiol regulates growth and fillet yield partially through miRNA-mediated changes in gene expression, thereby identifying new mechanisms affecting economically important traits.

Technical Abstract: 17-Estradiol (E2) is a steroid hormone that negatively affects muscle growth in rainbow trout, but the mechanism associated with this response is not fully understood. To better characterize the effects of E2 on muscle, we identified differentially regulated microRNAs (miRNAs) and muscle atrophy-related transcripts in juvenile rainbow trout exposed to E2. Small RNA-Seq analysis of E2-treated vs. control muscle identified 36 differentially expressed miRNAs including those known to be involved in myogenesis, cell cycle, apoptosis, and cell death. Some important myogenic miRNAs, such as miR-133 and miR-206, are upregulated while others like miR-145 and miR-499, are downregulated. Gene Ontology analysis of the target genes regulated by the miRNAs involved in atrophy and cell cycle indicates that E2 influence leads to expansion of quiescent myogenic precursor cell population to address atrophying mature muscle in rainbow trout during sexual development.