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ARS Home » Northeast Area » Frederick, Maryland » Foreign Disease-weed Science Research » Research » Publications at this Location » Publication #333136

Research Project: Identification, Characterization, and Biology of Foreign and Emerging Viral and Bacterial Plant Pathogens

Location: Foreign Disease-weed Science Research

Title: Comprehensive analysis of draft genomes of two closely related Pseudomonas syringae phylogroup 2b strains infecting mono and dicotyledon host plants

Author
item Sultanov, R - Moscow State University
item Arapidi, G - Moscow State University
item Vinogradova, S - Russian Academy Of Sciences
item Govorun, V - Moscow State University
item Luster, Douglas - Doug
item Ignatov, A - Russian Academy Of Sciences

Submitted to: BMC Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/29/2016
Publication Date: 12/29/2016
Citation: Sultanov, R.I., Arapidi, G.P., Vinogradova, S.V., Govorun, V.M., Luster, D.G., Ignatov, A.N. 2016. Comprehensive analysis of draft genomes of two closely related Pseudomonas syringae phylogroup 2b strains infecting mono and dicotyledon host plants. BMC Genomics. 17:1010. doi 10.1186/s12864-016-3358-y.

Interpretive Summary: In recent years, the damage caused by bacterial pathogens to major crops has been increasing worldwide. Pseudomonas syringae is a widespread bacterial species that infects almost all major crops. Different P. syringae strains use a wide range of biochemical mechanisms, including toxins and secreted proteins, that determine the specific nature of the pathogen's virulence. Two P. syringae strains, one isolated from dicots and one isolated from monocots, were selected for sequencing because they specialize on different host plant groups. We compared virulence factors in these and other available genomes of P. syringae to find genes responsible for the specialization of bacteria. We showed that the monocot strain belongs to the group that has been infecting monocots in Russia and USA for at least 50 years, while the dicot -infecting strain has relatively recently changed its host plant to dicots. The results can help to identify the genes that define specific nature of the virulence of P. syringae strains on their respective hosts.

Technical Abstract: In recent years, the damage caused by bacterial pathogens to major crops has been increasing worldwide. Pseudomonas syringae is a widespread bacterial species that infects almost all major crops. Different P. syringae strains use a wide range of biochemical mechanisms, including phytotoxins and effectors of the type III and type IV secretion systems, which determine the specific nature of the pathogen's virulence. Strains 1845 (isolated from dicots) and 2507 (isolated from monocots)were selected for sequencing because they specialize on different groups of plants. We compared virulence factors in these and other available genomes of phylogroup 2 to find genes responsible for the specialization of bacteria. We showed that strain 1845 belongs to the clonal group that has been infecting monocots in Russia and USA for a long time (at least 50 years). Strain 1845 has relatively recently changed its host plant to dicots. The results obtained by comparing the strain 1845 genome with the genomes of bacteria infecting monocots can help to identify the genes that define specific nature of the virulence of P. syringae strains.