Location: Reproduction ResearchTitle: Polymorphism in the intron 20 of porcine O-linked N-acetylglucosamine transferase Author
|Kim, Jong - Chonbuk National University|
|Nonneman, Danny - Dan|
|Kim, Doo-wan - National Institute Of Animal Science|
|Shin, Sangsu - Kyungpook National University|
Submitted to: Asian-Australasian Journal of Animal Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/22/2017
Publication Date: 8/1/2017
Publication URL: http://handle.nal.usda.gov/10113/5832870
Citation: Kim, J.G., Nonneman, D.J., Kim, D., Shin, S., Rohrer, G.A. 2017. Polymorphism in the intron 20 of porcine O-linked N-acetylglucosamine transferase. Asian-Australasian Journal of Animal Sciences. 30(8):1086-1092. doi:10.5713/ajas.17.0143.
Interpretive Summary: The enzyme O-linked N-acetylglucosamine transferase (OGT) modifies intracellular proteins by catalyzing the addition of a single sugar molecule, thereby regulating cellular activity. An insertion of a 281 basepair repetitive element in intron 20 of the porcine OGT gene was identified in European pig breeds, but not in Chinese pigs. OGT was mapped to the pig X chromosome and resides within quantitative trait loci (QTL) for follicle stimulating hormone (FSH), testis weight and backfat thickness. When pigs from an experimental cross of white composite and Meishan pigs were genotyped for the OGT insertion/deletion, the breed of origin for the X chromosome QTL was confirmed. This polymorphism provides a marker for identifying the breed of origin of this X chromosomal region in crossbred pigs containing Chinese and European pig breeds. More research is necessary to determine if the polymorphism affects reproduction or fatness in pigs.
Technical Abstract: Objective: O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of O-GlcNAc and GlcNAcylation has extensive crosstalk with phosphorylation to regulate signaling and transcription. Pig OGT is located near the region of chromosome X that affects follicle stimulating hormone level and testes size. The objective of this study was to find the variations of OGT between European and Chinese pigs. Methods: Pigs were tested initially for polymorphism in OGT among European and Chinese pigs by polymerase chain reaction and sequencing at the U.S. Meat Animal Research Center (USMARC). The polymorphism was also determined in an independent population of pigs including European and Chinese Meishan (ME) breeds at the National Institute of Animal Science (NIAS, RDA, Korea). Results: The intron 20 of OGT from European and Chinese pigs was 514 and 233 bp, respectively, in the pigs tested initially. They included 1 White composite (WC) boar and 7 sows (2 Minzu×WC, 2 Duroc [DU]×WC, 2 ME×WC, 1 Fengzing×WC) at USMARC. The 281-bp difference was due to an inserted 276-bp element and GACTT in European pigs. When additional WC and ME boars, the grandparents that were used to generate the 1/2ME×1/2WC parents, and the 84 boars of 16 litters from mating of 1/2ME×1/2WC parents were analyzed, the breeds of origin of X chromosome quantitative trait locus (QTL) were confirmed. The polymorphism was determined in an independent population of pigs including DU, Landrace, Yorkshire, and ME breeds at NIAS. OGT was placed at position 67 cM on the chromosome X of the USMARC swine linkage map. Conclusion: There was complete concordance with the insertion in European pigs at USMARC and NIAS. This polymorphism could be a useful marker to identify the breed of origin of X chromosome QTL in pigs produced by crossbreeding Chinese and European pigs.