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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Publications at this Location » Publication #331536

Research Project: Genetic Improvement of Hard Winter Wheat to Biotic and Abiotic Stresses

Location: Hard Winter Wheat Genetics Research

Title: Multiple minor QTLs are responsible for Fusarium head blight resistance in Chinese wheat landrace Haiyanzhong

Author
item CAI, JIN - Kansas State University
item WANG, SHAN - Kansas State University
item LI, TAO - Yangzhou University
item Bai, Guihua

Submitted to: PLOS ONE
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/13/2016
Publication Date: 9/27/2016
Publication URL: http://handle.nal.usda.gov/10113/63353
Citation: Cai, J., Wang, S., Li, T., Bai, G. 2016. Multiple minor QTLs are responsible for Fusarium head blight resistance in Chinese wheat landrace Haiyanzhong. PLoS One. 11(9):e0163292. doi:10.1371/journal.pone.0163292.

Interpretive Summary: Fusarium head blight (FHB) is a devastating disease in wheat. Cultivar resistance is one of the most effective strategies to minimize the disease damage. Chinese wheat landrace Haiyanzhong (HYZ) is highly resistant to FHB. Using DNA markers, we mapped 6 genes for FHB resistance in HYZ, and found that multiple minor genes on chromosomes 5A, 6B, 7D, 3B, 4B and 4D together can provide a high level of FHB resistance in wheat. Critical SNP markers linked to the genes on chromosomes 5A, 6B, and 7D were converted into breeder-friendly assays, and can be used for marker-assisted selection to pyramid these genes in wheat.

Technical Abstract: Fusarium head blight (FHB), caused by Fusarium graminearum Schwabe, is a devastatingve disease in wheat (Triticum aestivum L.). Use of host resistance is one of the most effective strategies to minimize the disease damage. Haiyanzhong (HYZ) is a Chinese wheat landrace that shows a high level of resistance to type II FHB resistance to spread within a spike (type II resistance). To map the quantitative trait loci (QTLs) in HYZ and identify markers tightly linked to the QTLs for FHB resistance, A a population of 172 recombinant inbred lines (RILs) from a cross between HYZ and Wheaton, a susceptible cultivar, was genotyped using simple sequence repeats (SSRs) and single-nucleotide polymorphisms (SNPs) derived from genotyping-by-sequencing (GBS), and evaluated for percentage of symptomatic spikelets (PSSs) per spike (type II resistance) in three greenhouse experiments. Six QTLs for type II resistance were identified in HYZ, indicating that multiple minor QTLs together can provide a high level of FHB resistance in wheat. The QTL with the largest effect on FHB resistance was mapped on the chromosome arm 5AS, and the other five were mapped on the chromosomes 6B, 7D, 3B, 4B and 4D. Critical SNPs linked to the QTLs on chromosomes 5A, 6B, and 7D were converted into KBioscience competitive allelice-specific PCR (KASP) assays, which can be used for marker-assisted selection (MAS) to pyramid these QTLs in wheat.