Location: Poisonous Plant ResearchTitle: Oocytes from small and large follicles exhibit equal development competence following goat cloning despite their differences in meiotic and cytoplasmic maturation Author
|Yang, Min - Utah State University|
|Hall, Justin - Utah State University|
|Fan, Zhiqiang - Utah State University|
|Regouski, Misha - Utah State University|
|Meng, Qinggang - Utah State University|
|Rutigliano, Heloisa - Utah State University|
|Stott, Rusty - Utah State University|
|Rood, Kerry - Utah State University|
|Polejaeva, Irena - Utah State University|
Submitted to: Theriogenology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/25/2016
Publication Date: 10/19/2016
Citation: Yang, M., Hall, J., Fan, Z., Regouski, M., Meng, Q., Rutigliano, H., Stott, R., Rood, K., Panter, K.E., Polejaeva, I. 2016. Oocytes from small and large follicles exhibit equal development competence following goat cloning despite their differences in meiotic and cytoplasmic maturation. Theriogenology. 86:2302-2311.
Interpretive Summary: Genetic engineering (cloning) of animals for research has been around for decades. The development of animal models to study disease conditions in humans requires novel approaches such as genetic engineering. Research to successfully create these animal models requires basic research to determine the optimum time for collection of cells such as oocytes and to determine the optimum timing for collection and transfer of genetic material from one cell to another or from one animal to another. This study further describes the importance of collecting maturing oocytes at the right time of maturation to optimize in vitro fertilization and cloning techniques.
Technical Abstract: Somatic cell nuclear transfer (SCNT) in animals has been around for nearly 20 years and has been successfully used for cloning of various livestock species. In this study, goat oocytes were collected from large follicles (>3mm) and small follicles (<3mm) to compare the success rate when used in goat SCNT. The results indicate no difference in pregnancy or birth rates when oocytes collected from large or small folluicles were used in goat SCNT.