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ARS Home » Southeast Area » Stoneville, Mississippi » Warmwater Aquaculture Research Unit » Research » Publications at this Location » Publication #331338

Research Project: Umbrella Project for Food Safety

Location: Warmwater Aquaculture Research Unit

Title: The role of Listeria monocytogenes cell wall surface anchor protein LapB in virulence, adherence, and intracellular replication

item REDDY, SWETHA - Mississippi State University
item AKGUL, ALI - Mississippi State University
item KARSI, ATTILA - Mississippi State University
item HOSSAM, ABDELHAMED - Mississippi State University
item WILLS, ROBERT - Mississippi State University
item LAWRENCE, MARK - Mississippi State University

Submitted to: Microbial Pathogenesis
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/22/2015
Publication Date: 12/25/2015
Citation: Reddy, S., Akgul, A., Karsi, A., Hossam, A., Wills, R., Lawrence, M. 2015. The role of Listeria monocytogenes cell wall surface anchor protein LapB in virulence, adherence, and intracellular replication. Microbial Pathogenesis. 92:19-25.

Interpretive Summary: We showed that Lmof2365_2117 (LapB) deletion mutant was not efficient in invading liver of mice. Also, the mutant exhibited impaired adherence to intestinal epithelial cells. Further, the mutant’s replication in intestinal epithelial cells and murine macrophages was lower compared to wild type. Finally, catfish fillet attachment of the mutant was significantly less than that of wild type.

Technical Abstract: Lmof2365_2117 is a Listeria monocytogenes putative cell wall surface anchor protein with a conserved domain found in collagen binding proteins. We constructed a deletion mutation in lmof2365_2117 in serotype 4b strain F2365, evaluated its virulence, and determined its ability to adhere and invade colonic epithelial cells and macrophages. In A/J mice, colonization of liver was significantly higher for F2365 than for F2365D2117. The ability of F2365D2117 to adhere to Caco-2 cells was significantly lower than F2365. The mutant also showed impaired ability to replicate in intestinal epithelial cell and murine macrophages relative to wild type F2365. Lmof2365_2117 contributed to L. monocytogenes attachment to catfish fillets. Because of its role in adherence to Caco-2 cells, we designated Lmof2365_2117 Listeria adhesion protein B (LapB). The carboxy terminus of LapB is similar to a domain in collagen binding proteins, but our results show that L. monocytogenes does not bind collagen.