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ARS Home » Southeast Area » Little Rock, Arkansas » Arkansas Children's Nutrition Center » Research » Publications at this Location » Publication #331101

Research Project: Impact of Early Dietary Factors on Child Development and Health

Location: Arkansas Children's Nutrition Center

Title: RNA-sequencing data analysis of uterus in ovariectomized rats fed with soy protein isolate,17B-estradiol and casein

Author
item RONIS, MARTIN - Arkansas Children'S Nutrition Research Center (ACNC)
item GOMEZ-ACEVEDO, H - Arkansas Children'S Nutrition Research Center (ACNC)
item BLACKBURN, M - Arkansas Children'S Nutrition Research Center (ACNC)
item CLEVES, M - Arkansas Children'S Nutrition Research Center (ACNC)
item SINGHAL, R - Arkansas Children'S Nutrition Research Center (ACNC)
item Badger, Thomas

Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 4/13/2016
Publication Date: 4/21/2016
Citation: Ronis, M.J., Gomez-Acevedo, H., Blackburn, M.L., Cleves, M.A., Singhal, R., Badger, T.M. 2016. RNA-sequencing data analysis of uterus in ovariectomized rats fed with soy protein isolate,17B-estradiol and casein. Data in Brief. 7:1491-1496. http://dx.doi.org/10.1016/j.dib.2016.04.033

Interpretive Summary: Although there is published data in both animal models and in young women that eating diets rich in soy are not harmful, concerns persist on the potential risks of soy-isoflavone consumption on female reproductive health during early development and in post-menopause. In this study, female rats were ovariectomized to induce menopause, and then fed diets containing either casein protein, soy protein, and compared to ovariectomized rodents receiving estradiol supplementation. A new technique for measuring gene expression called RNA-sequencing analysis was used to gather information on genes expressed in the uterus of these ovariectomized rats receiving casein protein diets with estradiol supplementation, or a soy protein diet supplemented with estradiol. Analysis of the data shows estradiol specific changes in gene expression in the casein protein diet that are not upregulated in the rats fed soy protein diets supplemented with estradiol. Understanding how these changes are related to the protein in these different diets is discussed in previous paper published by our group.

Technical Abstract: This data file describes the bioinformatics analysis of uterine RNA-seq data comparing genome wide effects of feeding soy protein isolate compared to casein to ovariectomized female rats age 64 days relative to treatment of casein fed rats with 5 ug/kg/d estradiol and relative to rats treated with estradiol and also fed soy protein isolate. Complete raw data files were deposited in the gene Expression Omnibus (GEO) at NCBI (http:/www.ncbi.nlm.nih.gov.geo/) under the GEO accession number GEO: GSE69819. Data presented here incudes a summary of the differential expression analysis with top 30 genes up- and down-regulated by soy protein isolate (SPI), estradiol (E2) and SPI+E2. Additional functional annotation analysis of KEGG pathways is also presented for each treatment, together with networks of interaction between those pathways. Further interpretation and discussion of this data can be found in the article "Uterine responses to feeding soy protein isolate and treatment with 17B-estradiol differ in ovariectomized female rats" Ronis et al. (2016) Nut Cancer; 67(6):943-953.