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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Infectious Bacterial Diseases Research » Research » Publications at this Location » Publication #330625

Research Project: PREVENTION AND CONTROL STRATEGIES FOR TUBERCULOSIS IN CATTLE AND WILDLIFE RESERVOIRS

Location: Infectious Bacterial Diseases Research

Title: Polymorphisms of twenty regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis

Author
item Bigi, Maria - University Of Buenos Aires
item Blaco, Federico - National Institute Of Agricultural Technology(INTA)
item Araujo, Flabio - Brazilian Ministry Of Agriculture
item Thacker, Tyler
item Zumarraga, Martin - National Institute Of Agricultural Technology(INTA)
item Cataldi, Angel - National Institute Of Agricultural Technology(INTA)
item Soria, Marcelo - University Of Buenos Aires
item Bigi, Fabriana - National Institute Of Agricultural Technology(INTA)

Submitted to: Microbiology and Immunology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/17/2016
Publication Date: 8/20/2016
Citation: Bigi, M.M., Blaco, F.C., Araujo, F.R., Thacker, T.C., Zumarraga, M.J., Cataldi, A.A., Soria, M.A., Bigi, F. 2016. Polymorphisms of twenty regulatory proteins between Mycobacterium tuberculosis and Mycobacterium bovis. Microbiology and Immunology. 60:552-560 doi: 10.1111/1348-0421.12402.

Interpretive Summary: Two members of the Mycobacteria family cause tuberculosis in humans and cattle, Mycobacterium tuberculosis and Mycobacterium bovis. The genomes of these tuberculosis-causing bacteria are over 99.95% identical. The genomes from 75 M. tuberculosis and 23 M. bovis isolates were sequenced and compared to each other. When M. tuberculosis was compared to M. bovis, differences in 20 control genes were discovered that may explain why these closely related bacteria survive and grow under different conditions.

Technical Abstract: Mycobacterium tuberculosis and Mycobacterium bovis are responsible for tuberculosis in humans or animals, respectively. Both species are closely related and belong to the Mycobacterium tuberculosis complex (MTC). M. tuberculosis is the most ancient species from which M. bovis and the other members of the MTC evolved. The genome of M. bovis is over 99.95% identical to that of M. tuberculosis but with seven deletions ranging in size from 1 to 12.7 kb. In addition, 1,200 single nucleotide mutations in coding regions distinguish M. bovis from M. tuberculosis. In this study we assessed 75 M. tuberculosis genomes and 23 M. bovis genomes to identify non-synonymous mutation in 202 coding sequences of regulatory genes between both species. We identified species-specific variants in 20 regulatory proteins and confirmed differential expression of hypoxia-related genes between M. bovis and M. tuberculosis. Key words: Mycobacterium bovis, Mycobacterium tuberculosis, regulators, polymophisms