|RAVELONANDRO, MICHEL - Institut National De La Recherche Agronomique (INRA)|
|BRIARD, PASCAL - Institut National De La Recherche Agronomique (INRA)|
Submitted to: Acta Horticulturae
Publication Type: Proceedings
Publication Acceptance Date: 7/15/2015
Publication Date: N/A
Technical Abstract: An important approach to controlling sharka disease caused by Plum pox virus (PPV) is the development of PPV resistant plants using small interfering RNAs (siRNA) technology. In order to evaluate siRNA induced gene silencing, we studied, based on knowledge of the PPV genome sequence, virus genome translation and replication. While little is known about the interference of the PPV genome in plums, the present manuscript provides evidence that interfering RNA acts with the dicer plant-enzyme complex to induce its degradation into small RNAs.