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ARS Home » Pacific West Area » Corvallis, Oregon » National Clonal Germplasm Repository » Research » Publications at this Location » Publication #330217
Title: Lessons learned from DNA-based tool development and use in a genebank

Author
item Bassil, Nahla
item Hummer, Kim
item Finn, Chad

Submitted to: Acta Horticulturae
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/10/2016
Publication Date: 4/20/2017
Citation: Bassil, N.V., Hummer, K.E., Finn, C.E. 2017. Lessons learned from DNA-based tool development and use in a genebank. Acta Horticulturae. 1156:25-36. doi: 10.17660/ActaHortic.2017.1156.4.

Interpretive Summary: In 2002, a molecular genetics laboratory was established at the United States Department of Agriculture, National Clonal Germplasm Repository (NCGR), in Corvallis, Oregon. This facility houses the US national strawberry collection. A main objective of the molecular genetics laboratory is to characterize these strawberry genetic resources at the DNA level. Plants from this collection have been analyzed using DNA-based tools for identity and parentage confirmation, diversity assessment, validation of markers associated with traits of economic importance, and phylogenetic studies. The development and application of different types of DNA-based markers in characterizing these strawberry genetic resources will be described. We will also discuss the lessons learned from these studies and future plans for molecular characterization in strawberry.

Technical Abstract: In 2002, a molecular genetics laboratory was established at the United States Department of Agriculture Agricultural Research Service (USDA-ARS), National Clonal Germplasm Repository (NCGR), in Corvallis, Oregon. This facility houses the US national genebank for strawberry (Fragaria L.). A main objective of the molecular genetics laboratory is to characterize Fragaria genetic resources. Plants from this collection have been analyzed using DNA-based tools for species identification, phylogenetic analyses, diversity assessment, identity and parentage confirmation, and development and validation of markers associated with traits of economic importance. The development and application of microsatellite or simple sequence repeat (SSR) markers, single nucleotide polymorphism (SNP) markers, and DNA sequences in characterizing these strawberry genetic resources will be described. We will also discuss the lessons learned from these studies and future plans for molecular characterization in Fragaria.