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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Sugarbeet and Potato Research » Research » Publications at this Location » Publication #329221

Research Project: Molecular Approaches for Improved Cercospora Leaf Spot Control in Sugarbeet

Location: Sugarbeet and Potato Research

Title: High-throughput SuperSAGE for gene expression analysis of Nicotiana tabacum - Rhizoctonia solani interaction

Author
item PORTIELES, ROXANA - Center For Genetic Engineering And Biotechnology (CIGB)
item OCHAGAVIA, MARIA - Center For Genetic Engineering And Biotechnology (CIGB)
item CANALES, EDUARDO - Center For Genetic Engineering And Biotechnology (CIGB)
item SILVA, YUSSUAN - Tobacco Research Institute
item CHACON, OSMANI - Tobacco Research Institute
item HERNANDEZ, INGRID - Center For Genetic Engineering And Biotechnology (CIGB)
item LOPEZ, YUNIOR - Center For Genetic Engineering And Biotechnology (CIGB)
item RODRIGUEZ, MAYRA - Center For Genetic Engineering And Biotechnology (CIGB)
item TERAUCHI, RYOHEI - Iwate Biotechnology Research Center
item BORROTO, CARLOS - Yucatan Center For Scientific Research
item SANTOS, RAMON - Bioplants Center
item Bolton, Melvin
item AYRA-PARDO, CAMILO - Center For Genetic Engineering And Biotechnology (CIGB)
item BORRAS-HIDALGO, ORLANDO - Center For Genetic Engineering And Biotechnology (CIGB)

Submitted to: BMC Research Notes
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/13/2017
Publication Date: 11/21/2017
Citation: Portieles, R., Ochagavia, M.E., Canales, E., Silva, Y., Chacon, O., Hernandez, I., Lopez, Y., Rodriguez, M., Terauchi, R., Borroto, C., Santos, R., Bolton, M.D., Ayra-Pardo, C., Borras-Hidalgo, O. 2017. High-throughput SuperSAGE for gene expression analysis of Nicotiana tabacum - Rhizoctonia solani interaction. BMC Research Notes. doi:10.1186/s13104-017-2934-9.
DOI: https://doi.org/10.1186/s13104-017-2934-9

Interpretive Summary: Plants are consistently under attack by potential pathogens. Knowledge of why certain varieties are susceptible to disease would be helpful for disease management. In this study, we used SuperSAGE technology to identify genes up- or down-regulated in Nicotiana tabacum in response to the plant pathogen Rhizoctonia solani. Over 10,000 genes were differentially expressed after infection, indicating a major reprogramming of the host as a consequence of disease. Many genes were categorized as defense, metabolism, or signal transduction-related. Several genes intricately involved with hormone production were also identified in N. tabacum. Virus-induced gene silencing was used to silence a gene in the salicylic acid pathway, which increased resistance in N. tabacum to R. solani. Taken together, the salicylic acid pathway contributes to disease development in N. tabacum, which will be important information for the development of disease management strategies in this pathosystem.

Technical Abstract: Plants are under continuous threat of infection by pathogens endowed with diverse strategies to colonize their host. Knowledge of plant susceptibility factors and the molecular processes involved in the infection process are critical for understanding plant-pathogen interactions. We used SuperSAGE technology combined with next-generation sequencing to generate 26 bp-Tag libraries from transcripts that were differentially up or downregulated during the compatible Nicotiana tabacum - Rhizoctonia solani interaction. A total of 11, 286 unique Tag sequences identified as differentially expressed were obtained after inoculation with R. solani compared to non-inoculated control samples. Based on gene ontology classification, several annotated UniTags corresponded to genes involved with defense response, metabolism and signal transduction. Quantitative PCR analysis was used to analyze and verify the regulation of a subset of these UniTags and for most of them a rapid and transient induction was confirmed. Analysis of the N. tabacum transcriptome during infection by R. solani using SuperSAGE has identified regulatory genes involved in several hormonal pathways in N. tabacum-specific mediated responses in plants. Silencing of an mRNA inducible by salicylic acid reduced susceptibility of N. tabacum to R. solani. We provide evidence that the salicylic acid pathway contributes to disease development caused by R. solani in tobacco. This will be useful information for future development of strategies toward management of diseases caused by this necrotrophic pathogen.