Location: Harry K. Dupree Stuttgart National Aquaculture Research CntrTitle: Evaluation of the antibody response to the LV-359-01 strain of flavobacterium columnare
Submitted to: Flavobacterium Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 9/25/2015
Publication Date: 10/27/2015
Citation: Lange, M.D., Brown, J.D., Farmer, B.D., Beck, B.H. 2015. Evaluation of the antibody response to the LV-359-01 strain of flavobacterium columnare [abstract]. 4th International conference on Members of the genus Flavobacterium, Auburn, Alabama. p. 70.
Technical Abstract: Flavobacterium columnare, the causative agent of columnaris disease produces substantial mortality worldwide among numerous freshwater farmed finfish species. As aquaculture production continues to increase the frequency of columnaris disease will only continue to rise. Add to this an increase in regulation of licensed therapeutants and resistance to antibiotics means that alternative methods of disease protection will be required. The goal of this study was to characterize the total antibody response in channel catfish that had survived an LV-359-01 F. columnare challenge. Antibodies against whole F. columnare antigens were assessed from two groups; control catfish (n=10) and catfish previously bath immunized with an attenuated LV-359-01 (n=10). Whole cell and extracellular bacterial products were electrophoresed, transferred and blotted with catfish serum. We identified shared antigens among the control and immunized groups of catfish. This would suggest that there may be conserved targets that stimulate catfish B-cell responses. The potential use of these bacterial protein targets for the production of recombinant vaccines will be discussed.