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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Cereal Crops Research » Research » Publications at this Location » Publication #328287

Research Project: Genetic Improvement of Durum and Spring Wheat for Quality and Resistance to Diseases and Pests

Location: Cereal Crops Research

Title: A new genetic linkage map of barley (Hordeum vulgare L.) facilitates genetic dissection of height and spike length and angle

Author
item Islamovic, Emir
item OBERT, DONALD - Limagrain Cereal Seeds
item OLIVER, REBEKAH - North Dakota State University
item MARSHALL, JULIET - University Of Idaho
item MICLAUS, KELCI - Sas Institute, Inc
item Hang, An
item Chao, Shiaoman
item Lazo, Gerard
item HARRISON, STEPHEN - Louisiana State University
item IBRAHIM, AMIR - Texas A&M University
item JELLEN, ERIC - Brigham Young University
item MAUGHAN, PETER - Brigham Young University
item BROWN, RYAN - General Mills, Inc
item JACKSON, ERIC - General Mills, Inc

Submitted to: Field Crops Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/1/2013
Publication Date: 12/9/2013
Publication URL: http://handle.nal.usda.gov/10113/62597
Citation: Islamovic, E., Obert, D.E., Oliver, R., Marshall, J.M., Miclaus, K.J., Hang, A., Chao, S., Lazo, G.R., Harrison, S.A., Ibrahim, A., Jellen, E.N., Maughan, P.J., Brown, R.H., Jackson, E.W. 2013. A new genetic linkage map of barley (Hordeum vulgare L.) facilitates genetic dissection of height and spike length and angle. Field Crops Research. 154:91-99.

Interpretive Summary: Plant height and spike length and angle are important agronomic traits in the production of barley (Hordeum vulgare L.) due to their strong correlations with lodging and disease susceptibility. The objective of this study was to identify genetic regions associated with each trait in a plant population derived from a cross of varieties Falcon and Azhul. Falcon is a spring six-row hulless feed barley with long spikes displaying obtuse angles, while Azhul is a spring dwarf, six-row hulless food barley with short spikes displaying acute angles. Quantitative trait loci (QTL) were detected on various chromosomes that influence plant height, and spike angle and length. We developed a barley genetic map that incorporated various types of molecular markers that breeders can use to detect these traits. By using comparative genomics, we identified possible candidate genes that could account for the traits, including some involved in gibberellic acid signaling and auxin- and ethylene-responsive pathways. This knowledge can be used in breeding for barley improvement.

Technical Abstract: Plant height and spike length and angle are important agronomic traits in the production of barley (Hordeum vulgare L.) due to strong correlations with lodging and disease. The objective of this study was to use QTL analysis to identify genetic regions associated with each trait in a recombinant inbred line (RIL) mapping population derived from a cross of Falcon by Azhul. Falcon is a spring six-row hulless feed barley with long spikes displaying obtuse angles, while Azhul is a spring dwarf, six-row hulless food barley with short spikes displaying acute angles. The population was genotyped using SNP, DArT and SSR markers and quantitative trait loci (QTL) were detected on chromosomes 2H (102.8 cM, spike length), 3H (89.2 cM, plant height and 38.2, spike angle and length), 4H (19.0 cM, spike length), and 5H (106.7 cM, spike angle). In conclusion, we developed a barley genetic map, which incorporated SNP, DArT and SSR markers, for detection of height and spike length and angle QTL. Three spike angle, one spike length and one plant height QTL were novel and by using comparative genomics we identified possible candidate genes involved in gibberellic acid signaling and auxin- and ethylene-responsive pathways. This knowledge can be used to generate suitable markers for barley breeding improvement.