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ARS Home » Northeast Area » Leetown, West Virginia » Cool and Cold Water Aquaculture Research » Research » Publications at this Location » Publication #328072

Research Project: Integrated Research Approaches for Improving Production Efficiency in Salmonids

Location: Cool and Cold Water Aquaculture Research

Title: Leucine and isoleucine reduce protein degradation in rainbow trout (Oncorhynchus mykiss) primary myoblast cultures

item Cleveland, Beth

Submitted to: Fish Biology Congress
Publication Type: Abstract Only
Publication Acceptance Date: 4/20/2016
Publication Date: 6/12/2016
Citation: Cleveland, B.M. 2016. Leucine and isoleucine reduce protein degradation in rainbow trout (Oncorhynchus mykiss) primary myoblast cultures. Fish Biology Congress. P339614.

Interpretive Summary:

Technical Abstract: Myogenic precursor cells were isolated from rainbow trout skeletal muscle and incubated in media containing 10% fetal bovine serum for 7 days, thereby differentiating into myoblasts. Rates of protein degradation were determined in response to minimal essential media (MEM) of various amino acid (AA) profiles. Incubation of cells in media without essential AAs increased protein degradation by 1.5-fold (P less than 0.05) compared to MEM containing a complete AA profile. This response was attenuated by 50% and 20% by addition leucine and isoleucine, respectively, into MEM lacking essential AAs. In contrast, cells incubated with MEM and essential AAs, minus leucine, exhibited protein degradation rates comparable to cells incubated in MEM without any essential AAs (P greater than 0.05), suggesting that leucine is primary regulator of protein turnover in muscle. With the exception of leucine and isoleucine, media that individually lacked other essential AAs did not produce a proteolytic response. These findings indicate the importance of understanding how leucine consumption, absorption, and metabolism interact to regulate protein retention and nutrient partitioning in salmonids.