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ARS Home » Southeast Area » Gainesville, Florida » Center for Medical, Agricultural and Veterinary Entomology » Chemistry Research » Research » Publications at this Location » Publication #328054

Title: Isolation, characterization, and expression analyses of tryptophan aminotransferase genes in a maize dek18 mutant

item BERNARDI, JAMILA - University Of Florida
item Li, Qin-Bao
item MAROCCO, ADRIANO - University Of Florida
item CHOUREY, PREM - University Of Florida

Submitted to: Genbank
Publication Type: Other
Publication Acceptance Date: 8/18/2015
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The dek18 mutant of maize has decreased auxin content in kernels. Molecular and functional characterization of this mutant line offers the possibility to better understand auxin biology in maize seed development. Seeds of the dek18 mutants are smaller compared to wild type seeds and the vegetative development of dek18 is delayed. dek18 genes related to the auxin biosynthesis ( ZmAlliinase/Tar1 and ZmTar3 ) were highly down-regulated in the mutant compared to the wild type. Structural analysis of these genes revealed that no nucleotide difference is present in dek18 homozygous seeds compared to Dek18, but ZmTar1 or 3 are barely expressed in dek18 homozygous seeds, it is proposed that the mutation responsible for the dek1 8 phenotype alters the upstream regulation of the auxin biosynthetic pathway. These cDNA and genomic DNA sequences were submitted to the NCBI database and the assigned accession numbers were KT447512, KT447513, KT447514 for ZmTar1 and ZmTar3 respectively.