Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2016
Publication Date: 12/1/2016
Citation: Ling, K., Li, R. 2016. A single nucleotide polymorphism at the right terminal region of Mexican papita viroid is a virulent determinant factor on tomato. Phytopathology. 106: S4.147. https://doi.org/10.1094/PHYto-106-12-S4.1.
Interpretive Summary: N/A
Technical Abstract: Mexican papita viroid (MPVd), a pospiviroid, causes serious disease outbreaks on greenhouse tomato in North America. Two dominant genotypes (MPV-S and MPV-M), sharing 93.8% sequence identity, incited striking different symptom expression (severe and mild) on tomato ‘Rutgers’. To determine genetic factor that is responsible for differential pathogenicity, using synthetic DNA approach, a series of chimeric infectious clones were generated through progressive replacement of each secondary structural domain in MPVd-M with their corresponding domain of MPVd-S. In plant inoculation assays, a chimera containing the Terminal Right domain of MPVd-S incited severe symptom on tomato similar to that induced by MPVd-S. The other three chimeras incited only mild symptoms similar to that of MPVd-M. A single nucleotide polymorphism in the Terminal Right region between MPVd-M (176U:A185) and MPVd-S (174G:C183) was identified as the virulent determinant factor for MPVd. This result supports variable pathogenicity functions in different pospiviroids, which may lead us to design a better strategy in managing this emerging viroid disease.