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ARS Home » Southeast Area » Oxford, Mississippi » Natural Products Utilization Research » Research » Publications at this Location » Publication #327246
Research Project: Discovery and Development of Natural Products for Pharmaceutical and Agrochemical Applications II

Location: Natural Products Utilization Research

Title: Bacillusin A, an antibacterial macrodiolide from Bacillus amyloliquefaciens

Author
item RAVU, RANGA - University Of Mississippi
item JACOB, MELISSA - University Of Mississippi
item CHEN, XIAOLONG - University Of Mississippi
item WANG, MEI - University Of Mississippi
item NASRIN, SHAMIMA - Auburn University
item KLOEPPER, JOSEPH - Auburn University
item LILES, MARK - Auburn University
item MEAD, DAVID - Lucigen Corporation
item KHAN, IKHLAS - University Of Mississippi
item LI, XING-CONG - University Of Mississippi

Submitted to: Journal of Natural Products
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/3/2015
Publication Date: 3/10/2015
Publication URL: https://handle.nal.usda.gov/10113/5498157
Citation: Ravu, R.R., Jacob, M.R., Chen, X., Wang, M., Nasrin, S., Kloepper, J.W., Liles, M.R., Mead, D.A., Khan, I.A., Li, X. 2015. Bacillusin A, an antibacterial macrodiolide from Bacillus amyloliquefaciens. Journal of Natural Products. 78:924-928. https://doi.org/10.1021/np500911k.
DOI: https://doi.org/10.1021/np500911k

Interpretive Summary: The search for new antibiotics against drug-resistant bacteria has led to the discovery of a novel macrodiolide from the Gram positive bacterium Bacillus amyloliquefaciens AP183. The compound showed potent antibacterial activities against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium. This study has laid a basis to explore the macrodiolide class for antibacterial drug development.

Technical Abstract: Bioassay-guided fractionation of the organic extracts of a Bacillus amyloliquefaciens strain (AP183) led to the discovery of a new macrocyclic polyene antibiotic, bacillusin A (1). Its structure was assigned by interpretation of NMR and MS spectroscopic data as a novel macrodiolide composed of dimeric 4-hydroxy-2-methoxy-6-alkenyl-benzoic acid lactones with conjugated pentaene-hexahydroxy polyketide chains. Compound 1 showed potent antibacterial activities against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus faecium with minimum inhibitory concentrations in a range of 0.6 to 1.2 'g/mL. The biosynthetic significance of this unique class of antibiotic compounds is briefly discussed.