|MANCHANDA, NANCY - Iowa State University|
|YE, LIANG - Dow Agro Sciences|
|WIMALANATHAN, KOKULAPALAN - Iowa State University|
|ROUNSLEY, STEVE - Dow Agro Sciences|
|WANG, KAN - Iowa State University|
|LAWRENCE-DILL, CAROLYN - Iowa State University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/16/2016
Publication Date: 3/17/2016
Citation: Manchanda, N., Andorf, C.M., Ye, L., Wimalanathan, K., Rounsley, S., Wang, K., Lawrence-Dill, C. 2016. Sequencing, assembly, and annotation of Maize B104 : A maize transformation resource. In: 58th Annual Maize Genetics Conference, March 17-20, 2016, Jacksonville, Florida. p. 92.
Technical Abstract: Maize transformation is complicated. Most lines are not readily cultured and transformed, making the germplasm available for genome engineering extremely limited. Developing a better understanding of the genomic regions responsible for differences in culturability and transformability would be a good start toward enabling the development of new transformation strategies. B104 was derived from the same populations as the B73 reference. The genomes are very similar (93% similar as defined by Romay et al. Genome Biology 2013), but B104 is readily transformed whereas B73 is not. The availability of the B104 genome sequence provides an opportunity to create the genomics data infrastructure that would enable other researchers to work with their own transformants more easily. This poster outlines our progress towards these goals.