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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #327221

Research Project: MaizeGDB: Enabling Access to Basic, Translational, and Applied Research Information

Location: Corn Insects and Crop Genetics Research

Title: Sequencing, assembly, and annotation of Maize B104 : A maize transformation resource

Author
item Manchanda, Nancy - Iowa State University
item Andorf, Carson
item Ye, Liang - Dow Agro Sciences
item Wimalanathan, Kokulapalan - Iowa State University
item Rounsley, Steve - Dow Agro Sciences
item Wang, Kan - Iowa State University
item Lawrence-dill, Carolyn - Iowa State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/16/2016
Publication Date: 3/17/2016
Citation: Manchanda, N., Andorf, C.M., Ye, L., Wimalanathan, K., Rounsley, S., Wang, K., Lawrence-Dill, C. 2016. Sequencing, assembly, and annotation of Maize B104 : A maize transformation resource. In: 58th Annual Maize Genetics Conference, March 17-20, 2016, Jacksonville, Florida. p. 92.

Interpretive Summary:

Technical Abstract: Maize transformation is complicated. Most lines are not readily cultured and transformed, making the germplasm available for genome engineering extremely limited. Developing a better understanding of the genomic regions responsible for differences in culturability and transformability would be a good start toward enabling the development of new transformation strategies. B104 was derived from the same populations as the B73 reference. The genomes are very similar (93% similar as defined by Romay et al. Genome Biology 2013), but B104 is readily transformed whereas B73 is not. The availability of the B104 genome sequence provides an opportunity to create the genomics data infrastructure that would enable other researchers to work with their own transformants more easily. This poster outlines our progress towards these goals.