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ARS Home » Midwest Area » Ames, Iowa » Corn Insects and Crop Genetics Research » Research » Publications at this Location » Publication #327215

Title: Investigating diversity and possible functions of G-quadruplexes in regulatory regions of maize genes

item HE, MINGZE - Iowa State University
item Andorf, Carson
item WALLEY, JUSTIN - Iowa State University
item WALIA, HARKAMAL - University Of Nebraska
item KOCH, KAREN - University Of Florida
item LIU, PENG - Iowa State University
item BASS, HANK - Florida State University
item LAWRENCE-DILL, CAROLYN - Iowa State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/16/2016
Publication Date: 3/17/2016
Citation: He, M., Andorf, C.M., Walley, J., Walia, H., Koch, K., Liu, P., Bass, H., Lawrence-Dill, C. 2016. Investigating diversity and possible functions of G-quadruplexes in regulatory regions of maize genes. In: 58th Annual Maize Genetics Conference, March 17-20, 2016, Jacksonville, Florida. p. 85.

Interpretive Summary:

Technical Abstract: G4-quadruplexes are reversible DNA structures that likely function in gene regulation, but exactly how they work is not known. G4 DNA can be predicted from sequence motifs such as the pattern G-G-G-N(1,7)-G-G-G-N(1,7)-G-G-G-N(1,7)-G-G-G-N(1,7). In the maize genome, G4 motifs were found to occupy non-random sites including antisense 5’ UTR hot spots in genes associated with low energy signaling and responses, including hypoxia, low sugar, and nutrient deprivation1. This enrichment suggests that maize G4 DNA may play a role in energy stress response. We are conducting analyses that seek to determine whether and how genes harboring G4 elements: (1) contribute to plant developmental processes and stress response, (2) vary in constitution across diverse germplasm, and (3) could be combined (through plant breeding or gene editing) to modulate the stress response. Here we describe our initial results and outline next steps for these investigations.