Location: Renewable Product Technology ResearchTitle: Tyrosol-based liposomal behavior: Size, z-potential, TEM, QCM-D and fluorescence analysis
Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 8/21/2016
Publication Date: 8/21/2016
Citation: Evans, K.O., Compton, D.L., Appell, M.D. 2016. Tyrosol-based liposomal behavior: Size, z-potential, TEM, QCM-D and fluorescence analysis [abstract]. American Chemical Society. Poster AGFD#78.
Technical Abstract: In our continued efforts to create biobased antioxidants for the food industry, we have used phospholipase D from Streptomyces sp. to create hydroxytyrosol and tyrosol phospholipids. Extrusion methods proved that both hydroxytyrosol phospholipids and tyrosol phospholipids each formed liposomes that were pH-dependent in size. Z-potential analysis demonstrated that the surface charge was well below the threshold of -25 mV for stable nanoparticles. Transmission electron microscopy (TEM) analysis demonstrated that both types of liposomes were spherical and had a single bilayer characteristic. Quartz crystal microbalance with dissipation monitoring (QCMD) analysis demonstrated that both types of liposomes could form supported bilayers under proper cation (Ca2+) and pH conditions. Fluorescence anisotropy measurements indicate that melting temperatures of both lipids were below 4 °C, suggesting that adsorption behavior and liposome formation was limited by electrostatic interactions and not gel-state formation of phospholipids.