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ARS Home » Pacific West Area » Pullman, Washington » Plant Germplasm Introduction and Testing Research » Research » Publications at this Location » Publication #326972

Research Project: Management of Plant Genetic Resources and Associated Information

Location: Plant Germplasm Introduction and Testing Research

Title: Genome wide association study using the ICARDA Lentil Reference set and agronomic data

Author
item Rajendran, K. - International Center For Agricultural Research In The Dry Areas (ICARDA)
item Zheng, P. - Washington State University
item Kumar, S. - International Center For Agricultural Research In The Dry Areas (ICARDA)
item Main, D. - Washington State University
item Coyne, Clarice - Clare
item Saha, G. - Washington State University
item Amin, N. - Washington State University
item Bett, K. - University Of Saskatchewan
item Mcgee, Rebecca

Submitted to: Food Legume Research International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/5/2016
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Lentil (Lens culinaris Medik.) is the world’s fifth most important pulse crop. It is self-pollinated diploid (2n=14) and has a relatively large genome size of approximately 4Gb. Next generation sequencing (NGS) technology was used to genotype the ICARDA Lentil Reference set using the two-enzyme (PstI, MspI) genotyping-by-sequencing (GBS) method described by Poland. This method has been shown to be capable of discovering and genotyping thousands of markers across the lentil genome. We genotyped 192 accessions from the lentil reference collection plus stress responsive lines derived from the diverse lentil collection held by ICARDA. The software package “Stacks” and in-house scripts were used to call 10,050 high quality single nucleotide polymorphisms (SNPs). GWAS with the collection was tested using historic agronomic data. Associated markers will be reported and the potential of expanding the population and phenotypic experiments to improve association precision will be discussed.