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ARS Home » Midwest Area » Peoria, Illinois » National Center for Agricultural Utilization Research » Renewable Product Technology Research » Research » Publications at this Location » Publication #326615

Research Project: New Biobased Products and Improved Biochemical Processes for the Biorefining Industry

Location: Renewable Product Technology Research

Title: Production of novel antistreptococcal liamocins by fermentation of agricultural biomass

item Leathers, Timothy
item Price, Neil
item Bischoff, Kenneth
item MANITCHOTPISIT, PENNAPA - Illinois State University

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/24/2016
Publication Date: 7/25/2016
Citation: Leathers, T.D., Price, N.P., Bischoff, K.M., Manitchotpisit, P. 2016. Production of novel antistreptococcal liamocins by fermentation of agricultural biomass [abstract]. Society for Industrial Microbiology and Biotechnology. Poster P20, Paper 32367.

Interpretive Summary:

Technical Abstract: Liamocins are unique heavier-than-water “oils” produced by certain strains of the fungus Aureobasidium pullulans. Liamocins have antibacterial activity with specificity for Streptococcus sp. Previous studies reported that liamocin yields were highest from strains of A. pullulans belonging to phylogenetic clades 8, 9, and 11, cultured on a specific basal medium containing sucrose. In this study, 26 strains of A. pullulans from these clades were examined for the first time for production of liamocins from agricultural biomass substrates. After seven days of growth, four strains produced at least 1.5 g of liamocins/L from medium containing 5% (w/v) alkaline hydrogen peroxide-pretreated wheat straw as a sole carbon source. Liamocins produced from wheat straw were free of the melanin contamination common in sucrose-grown cultures. Furthermore, MALDI-TOF MS analysis showed that liamocins produced from wheat straw were underacetylated, resulting in higher proportions of the mannitol-B1 species of liamocin, which has the highest biological activity against Streptococcus sp. Production of liamocins from low-cost agricultural biomass might be particularly appropriate for bulk agricultural applications, such as in dairy cattle dips for prevention of mastitis caused by Streptococcus sp.