|Le Guyon, Soazig|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/2016
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: Clinical studies rely heavily on mouse models of infection. Precise identification and control of contaminating pathogens that circulate in mouse colonies is an important task. Over the past decade, there have been several reports documenting the isolation of Bordetella spp. from purported pathogen-free C57Bl/6 mice housed in various facilities in the United States, Europe and Asia. To accurately speciate Bordetella isolates from mice, we sequenced the genomes of three and compared them with genome sequences of other Bordetella species. We reconstructed whole-genome-based and 16S-rRNA-gene-based phylogenies for all Bordetella spp. sequenced to date. We determined estimated DNA-DNA hybridization (eDDH) values and average nucleotide identity (ANI) scores. Metabolic profiles and antimicrobial sensitivities were assayed with Biolog PM1 and PM2 tests, Biolog GENIII, API20 NE system, and Epsilometer tests. The ability of each of the three isolates to colonize the lungs of mice was assessed using a previously described murine model of infection. Each of the two phylogenies placed the three murine isolates into a distinct monophyletic cluster, most closely related to B. hinzii. Furthermore, distinctly different eDDH, ANI, gene content, metabolic profiles, and host specificity all provide compelling evidence for delineation of murine isolates from the B. hinzii group. Therefore, we designate these murine isolates as B. pseudohinzii spp. nov. Our study further provides a means to easily and precisely identify B. pseudohinzii spp. nov., based on either the presence of type II CRISPR-Cas genes that are otherwise absent in all other bordetellae or a unique pattern of mutations observed in their 16S rRNA genes.