Location: Cotton Chemistry and Utilization ResearchTitle: Hydrogen peroxide production from fibrous pectic cellulose analogs and effect on dermal fibroblasts
|Edwards, Judson - Vince|
|YAGER, DORNE - Virginia Commonwealth University|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/19/2016
Publication Date: N/A
Technical Abstract: Naturally derived products with folklore remedies have in recent years been reconsidered for their benefit to wound healing i.e., honey’s application to chronic wound dressing products. Similarly, we have undertaken an evaluation of Fibrous pectin-cellulose (FPC) (cellulose blended with primary cell wall pectin at 2 percent by weight of pectin), which is a material made from naturally occurring plant fibers. FPC is a mixture of polysaccharides with a low percent by weight of pectin-based primary cell wall and lipid components attached to absorbent cellulose I (microfibrillar bundles). FPC generates hydrogen peroxide associated with cell signaling and enhanced proliferation. Here we assess hydrogen peroxide levels in a variety of FPC analogs and plant phenotypes. Methods: FPC sourced from a variety of genetic plant fiber lines was utilized to prepare nonwoven dressings through a hydroentanglement process using a Fleissner waterjet protocol. Properties including water vapor transmission rates, absorption capacity, and wicking were shown to conform with dressing guidelines. Amplex Red and Formazan (used in the cell incubation) were employed to measure H2O2 in FPC dressings and starting material. FPC analogs were incubated overnight in serum free media, the centrifuged supernatants from the analogs were mixed with 2% serum, applied to dermal fibroblast cell lines, and the results assessed following overnight incubation. Results: A variety of FPC analogs/phenotypes and one commercial dressing demonstrated hydrogen peroxide production from 5-40 micromolar as measured with Amplex Red. Whereas, Formazan in the cell proliferation assay revealed 12-59 micromolar H2O2 levels in three analogs. It was found that 50mg/mL of FPC is not significantly cytotoxic to dermal fibroblasts, and there were some morphology changes in fibroblasts after overnight incubation warranting longer incubation. Conclusion: FPC analogs can be prepared through a process that retains the active constituents of hydrogen peroxide production in a range associated with cell proliferation.