|SELVARAJ, VIJAY ANAND RAJ - Foreign Agricultural Service (FAS, USDA)|
|MAHESHWARI, YOGITA - Foreign Agricultural Service (FAS, USDA)|
|BEDERSKI, KLAUS - Topara Nursery|
|HAJERI, SUBHAS - Central California Tristeza Eradication Agency|
|Yokomi, Raymond - Ray|
Submitted to: Conference of International Organization of Citrus Virologists
Publication Type: Abstract Only
Publication Acceptance Date: 3/22/2016
Publication Date: 4/11/2016
Citation: Selvaraj, V., Maheshwari, Y., Bederski, K., Hartung, J.S., Hajeri, S., Yokomi, R.K. 2016. Molecular characterization of Peruvian Citrus tristeza virus isolates based on 3’UTR sequences. Conference of International Organization of Citrus Virologists. p.58.
Technical Abstract: Citrus in Peru was decimated by quick decline and stem pitting strains of Citrus tristeza virus (CTV). Commercial citrus production in Peru is being restored by use of CTV cross-protection. To characterize the predominant CTV strains involved, Peruvian CTV isolates from “protected” and “non-protective” trees were imported and propagated in a quarantine facility in Beltsville, MD. Isolates were examined by serology, multiple molecular marker, single-stranded conformational polymorphism, and Taqman-based Revere Transcription quantitative PCR assays but strain differentiation was unclear. Therefore, 14 Peruvian CTV isolates were screened based on sequences derived from 3’ Untranslated Region (UTR) degenerate primers since 3’UTR sequences are conserved in the CTV genome. Phylogenetic analysis of Peruvian isolates with 56 known CTV isolates from NCBI based on the 3’UTR region showed that 11 Peruvian isolates were in the VT clade and 3 were in the RB clade. This data was confirmed with additional phylogenetic analysis of selected Peruvian isolates based on 9 ORFs (p6 to p23). In summary, screening of CTV genotypes using 3’UTR sequences was more precise than other techniques and is being used to characterize putative CTV cross-protective isolates and its phenotype.