|WANG, JING - Henan Agricultural University|
|ZHONG, JIA-QING - Henan Agricultural University|
|CHEN, JUN-FENG - Henan Agricultural University|
|GAO, BIN-WEN - Henan Agricultural University|
|HUA, LIU-SHUAI - Henan Agricultural University|
|Li, Congjun - Cj|
|SHI, ZHI-HAI - Henan Agricultural University|
|BAI, XIAN-XIAO - Henan Agricultural University|
|SHENG, WEI-DONG - Henan Agricultural University|
|XING, BAO-SONG - Henan Agricultural University|
Submitted to: Biomed Central (BMC) Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/27/2017
Publication Date: 7/19/2017
Citation: Wang, J., Zhong, J., Chen, J., Gao, B., Hua, L., Li, C., Shi, Z., Bai, X., Sheng, W., Xing, B. 2017. Identification and characterization of long non-coding RNAs in subcutaneous adipose tissue from castrated and intact full-sib pair Huainan male pigs. Biomed Central (BMC) Genomics. 18:542. https://doi.org/10.1186/s12864-017-3907-z.
Interpretive Summary: Testosterone deficiency is associated with obesity in humans. It has been proven that long non-coding RNAs (lncRNAs) regulate adipose tissue metabolism. We used neutered male pigs as animal model to study testosterone deficiency-induced fat deposition and the function roles of incRANs. Our results suggested that lncRNAs and their target genes might play an important role in the castration-induced fat deposition and provide a new therapeutic target for combatting testosterone deficiency-related obesity.
Technical Abstract: Testosterone deficiency is associated with obesity in humans. It has been proven that long non-coding RNAs (lncRNAs) regulate adipose tissue metabolism; therefore, we first study the role of lncRNAs on testosterone deficiency-induced fat deposition using castrated male pigs as the model animal. The results showed that castration reduced serum testosterone but increased body fatness-related traits (serum triglyceride levels, backfat thickness, intramuscular fat content, and adipocyte size). Meanwhile, 343 lncRNAs from subcutaneous adipose tissue were identified, including nine up- and nine down-regulated (q-value<0.05, |log2FoldChange|>1) lncRNAs. Functional analysis indicated that these 18 lncRNAs and their target genes are involved in fatty acid, insulin, and the adipocytokine signaling pathway. We further analyzed the features of a conserved mouse lncRNA gene ENSMUSG00000100005 and found it was enriched in the cell nucleus and associated with the Nuclear Receptor Subfamily 2 Group F Member 2 (NR2F2) gene. In 3T3-L1 cells, differentiation down-regulated their expression, but dihydrotestosterone (DHT) significantly up-regulated their expression in a concentration-dependent manner (P<0.05). These results suggested that lncRNAs and their target genes might play an important role in the castration-induced fat deposition and provide a new therapeutic target for combatting testosterone deficiency-related obesity.