|SANDERS, MELANIE - Former ARS Employee|
|MCPARTLIN, D - Dublin City University|
|MORAN, K - Dublin City University|
|GUO, Y - Zhejian University|
|EECKHOUT, M - Ghent University|
|O'KENNEDY, R - Dublin City University|
|DE SAEGER, S - Ghent University|
Submitted to: Toxins
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/29/2016
Publication Date: 4/11/2016
Citation: Sanders, M., McPartlin, D., Moran, K., Guo, Y., Eeckhout, M., O'Kennedy, R., De Saeger, S., Maragos, C. 2016. Comparison of enzyme-linked immunosorbent assay, surface plasmon resonance and biolayer interferometry for screening of deoxynivalenol in wheat and wheat dust. Toxins. 8(4):103.
Interpretive Summary: Deoxynivalenol (DON) is a toxin produced by certain fungi that cause Fusarium Head Blight, a disease of substantial economic significance worldwide. In order to facilitate on-site DON screening in wheat, an aqueous sample preparation for wheat and its corresponding wheat dust was developed. This sample preparation was coupled to enzyme-linked immunosorbent assay (ELISA) (developed at Ghent University) and sensor-based immunoassays, namely surface plasmon resonance (SPR) (developed at Dublin City University) and biolayer interferometry (BLI) (developed at ARS National Center for Agricultural Utilization Research (NCAUR) in Peoria, IL). Results suggest that the ELISA and BLI methods can be applied as a sensitive technique for rapid screening of DON in wheat and wheat dust. The analysis of dust is a fast and easy-to-use technique which can be performed on-site.
Technical Abstract: A sample preparation method was developed for the screening of deoxynivalenol (DON) in wheat and wheat dust. Extraction was carried out with water and was successful due to the polar character of DON. For detection, an enzyme-linked immunosorbent assay (ELISA) was compared to the sensor-based techniques of surface plasmon resonance (SPR) and biolayer interferometry (BLI) in terms of sensitivity, affinity and matrix effect. The matrix effects from wheat and wheat dust using SPR were too high to further use this screenings method. The preferred ELISA and BLI methods were validated according to the criteria established in Commission Regulation 519/2014/EC and Commission Decision 2002/657/EC. A small survey was executed on 16 wheat lots and their corresponding dust samples using the validated ELISA method. A linear correlation (r = 0.889) was found for the DON concentration in dust versus the DON concentration in wheat (LOD wheat: 233 _g/kg, LOD wheat dust: 458 _g/kg).